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T4000

Sigma-Aldrich

Tetranitroblue tetrazolium chloride

Synonym(s):

3,3′-(3,3′-Dimethoxy-4,4′-biphenylene)bis[2,5-bis(p-nitrophenyl)-2H-tetrazolium chloride], TNBT, Tetranitrotetrazolium blue chloride

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About This Item

Empirical Formula (Hill Notation):
C40H28Cl2N12O10
CAS Number:
1184-43-6
Molecular Weight:
907.63
Beilstein:
3897475
EC Number:
214-665-1
MDL number:
MFCD00036338
UNSPSC Code:
12171500
PubChem Substance ID:
24900185
NACRES:
NA.47

Assay

≥90%

form

powder

mp

~170 °C (dec.)

solubility

water: 1 mg/mL, clear to hazy, yellow

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

[Cl-].[Cl-].COc1cc(ccc1-[n+]2nc(nn2-c3ccc(cc3)[N+]([O-])=O)-c4ccc(cc4)[N+]([O-])=O)-c5ccc(c(OC)c5)-[n+]6nc(nn6-c7ccc(cc7)[N+]([O-])=O)-c8ccc(cc8)[N+]([O-])=O

InChI

1S/C40H28N12O10.2ClH/c1-61-37-23-27(7-21-35(37)47-43-39(25-3-9-31(10-4-25)49(53)54)41-45(47)29-13-17-33(18-14-29)51(57)58)28-8-22-36(38(24-28)62-2)48-44-40(26-5-11-32(12-6-26)50(55)56)42-46(48)30-15-19-34(20-16-30)52(59)60;;/h3-24H,1-2H3;2*1H/q+2;;/p-2

InChI key

VCESGVLABVSDRO-UHFFFAOYSA-L

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Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H350

Hazard Classifications

Carc. 1B

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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C J van Noorden et al.
Histochemistry, 75(4), 493-506 (1982-01-01)
A sensitive cytochemical staining method for glucose-6-phosphate dehydrogenase activity in individual human erythrocytes is described. This staining method can be used for the rapid routine discrimination of patients with a deficiency of the enzyme in its homozygote or heterozygote form
W M Frederiks et al.
Experimental and molecular pathology, 41(1), 119-125 (1984-08-01)
A method has been developed for quantitative analysis of necrotic regions in lobes of the rat liver 24 hr after temporary ischemia. Essentially the method consists of cutting the liver lobes into 2-mm-thick slices by means of a specially developed
C J Van Noorden et al.
The Histochemical journal, 12(6), 669-685 (1980-11-01)
The cytochemical determination of the activity of glucose-6-phosphate dehydrogenase (G6PDH) with tetranitro blue tetrazolium (TNBT) was studied with model films of polyacrylamide gel incorporating purified enzyme. This model system enabled a quantitative study to be made of different parameters involved
R G Butcher
Histochemistry, 81(6), 567-572 (1984-01-01)
The reaction velocity of glucose-6-phosphate dehydrogenase has been quantified by continuous monitoring on a Vickers microdensitometer of the reaction product as it formed in sections of different thickness of rat tracheal epithelium. Reaction velocity was directly proportional to section thickness
C J Van Noorden et al.
The Histochemical journal, 13(2), 187-206 (1981-03-01)
This paper deals with the progress made over the last few years in our understanding of enzyme cytochemical staining methods as studied using a fundamental approach with the aid of a model system of thin gel films. Although model films
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