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Key Documents

R9379

Sigma-Aldrich

Rhodamine B isothiocyanate–Dextran

average mol wt ~70,000

Synonym(s):

RITC-Dextran

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About This Item

MDL number:
UNSPSC Code:
12171501
NACRES:
NA.56

mol wt

average mol wt ~70,000

Quality Level

extent of labeling

0.002-0.015 mol RITC per mol glucose

storage temp.

2-8°C

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General description

Rhodamine B is a thiol-reactive and photostable fluorescent dye.

Application

Rhodamine B isothiocyanate-Dextran has been used:
  • to label A, CI-M6PR-GFP, Lamp1-CFP-expressing wild type mouse embryonic fibroblast (MEF) cells
  • in endosome/phagosome fusion assay to determine the rate of fusion of endosomes
  • as a component in poration medium to monitor Hela cell uptake

Dextran labeled with rhodamine B isothiocyanate for possible use in perfusion studies in animals.

Biochem/physiol Actions

Rhodamine B stains the tissue in red-violet color and emits a bright red color when fluoresced. It is highly preferred for marking insects.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mohammad A Kaisar et al.
Redox biology, 13, 353-362 (2017-06-25)
Recently published in vitro and in vivo findings strongly suggest that BBB impairment and increased risk for stroke by tobacco smoke (TS) closely resemble that of type-2 diabetes (2DM) and develop largely in response to common key modulators such oxidative
Edward Harris et al.
Molecular biology of the cell, 13(2), 656-669 (2002-02-21)
Chediak-Higashi syndrome is a genetic disorder caused by mutations in a gene encoding a protein named LYST in humans ("lysosomal trafficking regulator") or Beige in mice. A prominent feature of this disease is the accumulation of enlarged lysosome-related granules in
Xiuqing Gong et al.
Lab on a chip, 10(19), 2622-2627 (2010-08-07)
We report a simple, low-cost and detachable microfluidic chip incorporating easily accessible paper, glass slides or other polymer films as the chip materials along with adhesive wax as the recycling bonding material. We use a laser to cut through the
Shikha Prasad et al.
Redox biology, 12, 58-69 (2017-02-18)
Cigarette smoking (CS) is associated with vascular endothelial dysfunction in a causative way primarily related to the TS content of reactive oxygen species (ROS), nicotine, and inflammation. TS promotes glucose intolerance and increases the risk of developing type-2 diabetes mellitus
Sidi A Bencherif et al.
Biomacromolecules, 10(9), 2499-2507 (2009-08-29)
A nanostructured hyaluronic acid (HA) hydrogel was prepared by a combination of atom transfer radical polymerization (ATRP) and Michael-type addition reaction. Biodegradable POEO(300)MA-co-PHEMA nanogels with pendent hydroxy groups were prepared by activators generated by electron transfer ATRP in cyclohexane inverse

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