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P9541

Sigma-Aldrich

Potassium chloride

for molecular biology, ≥99.0%

Synonym(s):

KCl

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About This Item

Linear Formula:
KCl
CAS Number:
7447-40-7
Molecular Weight:
74.55
EC Number:
231-211-8
MDL number:
MFCD00011360
UNSPSC Code:
12352302
PubChem Substance ID:
24899042
NACRES:
NA.31

grade

for molecular biology

Quality Level

200

Assay

≥99.0%

form

powder

mp

770 °C (lit.)

density

1.98 g/mL at 25 °C (lit.)

cation traces

Fe: ≤3 ppm
heavy metals (as Pb): ≤5 ppm

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

SMILES string

[Cl-].[K+]

InChI

1S/ClH.K/h1H;/q;+1/p-1

InChI key

WCUXLLCKKVVCTQ-UHFFFAOYSA-M

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General description

Potassium chloride (KCl) is useful in studies of ion transport and potassium channels. KCl is known to be used in the crystallization of histone core octamers.

Application

Potassium chloride has been used:
  • as a component of Danieau medium and also used in the preparation of phosphate buffer saline
  • in the preparation of electrolyte solution for electrochemical impedance spectroscopy and cyclic voltammetry measurements
  • as a component of HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)-buffered Tyrode′s solution

May be used for the preparation of phosphate buffered saline, and for the extraction and solubilization of proteins.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Reconstitution of nucleosome core particles from recombinant histones and DNA
Test, 375(20), 23-44 (2003)
Fluid osmolarity acutely and differentially modulates lymphatic vessels intrinsic contractions and lymph flow
Solari E, et al.
Frontiers in Physiology, 9(1), 871-871 (2018)
Electrochemical impedance spectroscopic technique with a functionalized microwire sensor for rapid detection of foodbornepathogens
Lu L, et al.
Biosensors And Bioelectronics, 42(1), 492-495 (2013)
Diffusion through channel derivatives of the Escherichia coli FhuA transport protein
Braun M, et al.
European Journal of Biochemistry, 269(20), 4948-4959 (2002)
High-resolution in situ hybridization to whole-mount zebrafish embryos.
Thisse C and Thisse B
Nature Protocols, 3(1), 59-69 (2008)
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Protocols

Restriction Enzyme Cloning Manual Buffer Recipes

TE Buffer; Elution Buffer; 10x Ligation Buffer; 0.5 M PIPES Buffer; Inoue Transformation Buffer

RNAi In Situ

In Situ Hybridization of Whole-Mount Mouse Embryos with RNA Probes: Hybridization, Washes, and Histochemistry. This is a protocol describing how to perform in situ hybridization on whole mouse embryos. Here we describe the hybridization procedure, and the localization of the DIG-labeled RNA using a conjugate of anti-DIG Fab antibody and calf intestinal alkaline phosphatase. Enzyme activity of the reporter is detected by a color reaction, resulting in the formation of a water-insoluble purple/blue precipitate. Manipulating the Mouse Embryo - Third Edition

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