P5381
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae)
Type III, ammonium sulfate suspension, ≥400 units/mg protein (biuret)
Synonym(s):
D-Glucose-6-phosphate ketol-isomerase, PGI, Phosphosaccharomutase
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About This Item
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biological source
bakers yeast
type
Type III
form
ammonium sulfate suspension
specific activity
≥400 units/mg protein (biuret)
mol wt
tetramer 119,500 Da±600
concentration
≥3 mg/mL protein Biuret
technique(s)
activity assay: suitable
color
white
Protein ID accession no.
UniProt accession no.
storage temp.
2-8°C
Gene Information
yeast ... PGI1(852495)
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General description
Research area: Cell Signaling
Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.
Phosphoglucose Isomerase ubiquitous multifunctional cytosolic protein. This dimeric protein has a molecular weight of approximately 119,500 ± 600 and is composed of four subunits that are nearly identical.
Application
Phosphoglucose Isomerase from baker′s yeast (S. cerevisiae) has been used:
- to convert fructose-6-phosphate to glucose-6-phosphate during the enzymatic measurement of serum mannose levels.
- as one of the enzymes in the series of four enzyme-coupled reactions to measure phosphomannomutase (PMM) activity.
- to convert fructose to glucose while evaluating fructose content in kudzu roots.
Biochem/physiol Actions
Phosphoglucose Isomerase(PGI) is an enzyme crucial for the interconversion of D-glucose 6-phosphate and D-fructose 6-phosphate. PGI is responsible for the second step of glycolysis and is involved in glucogenesis and pentose phosphate pathway (PPP). It is highly conserved in bacteria and eukaryotes. PGI plays a vital role in the survival of A. fumigatus and demonstrates diverse functions that encompass development, biosynthesis of cell wall glucan, and virulence.Phosphoglucose Isomerase functions as an isomerase, neuroleukin, autocrine motility factor, and a differentiation and maturation mediator.
Unit Definition
One unit will convert 1.0 μmole of D-fructose 6-phosphate to D-glucose 6-phosphate per min at pH 7.4 at 25 °C.
Physical form
Crystalline suspension in 2.6 M (NH4)2SO4 containing 0.1 mM magnesium acetate, pH 7.0
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
10 - Combustible liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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S L Lowe et al.
The Journal of biological chemistry, 250(1), 94-99 (1975-01-10)
Bakers' yeast phosphoglucose isomerase was studied by both chemical and physical methods to determine its submit structure. Gel filtration in 6 M guanidine HCl as well as acrylamide gel electrophoresis of sodium dodecyl sulfatedentured phosphoglucose isomerase showed two speices corresponding
Kudzu [Pueraria montana (Lour.) Merr. Variety lobata: A new source of carbohydrate for bioethanol production
Sage RF, et al.
Biomass and Bioenergy, 33, 57-61 (2009)
J Pozueta-Romero et al.
FEBS letters, 291(2), 233-237 (1991-10-21)
The standardized enzyme coupling method for assaying sucrose synthase activities in the direction of sucrose cleavage was reexamined using enzyme preparations from cultured cells of sycamore (Acer pseudoplatanus L.) and spinach leaves (Spinacea oleracea). Both ATP and Tris, commonly utilized
B K Campbell et al.
Reproduction (Cambridge, England), 140(5), 721-732 (2010-08-19)
Glucose is a critical metabolic fuel in most mammals although many foodstuffs also contain high levels of the monosaccharides, galactose and fructose. The aims of this work were to determine the insulin response to challenges of these sugars (experiment 1)
Johannes Yayo et al.
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The native ability of Clostridium thermocellum to efficiently solubilize cellulose makes it an interesting platform for sustainable biofuel production through consolidated bioprocessing. Together with other improvements, industrial implementation of C. thermocellum, as well as fundamental studies into its metabolism, would
Articles
Instructions for working with enzymes supplied as ammonium sulfate suspensions
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