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P5280

Sigma-Aldrich

Sodium pyruvate

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Synonym(s):

α-Ketopropionic acid sodium salt, 2-Oxopropanoic acid sodium salt, Pyruvic acid sodium salt

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About This Item

Linear Formula:
CH3COCOONa
CAS Number:
113-24-6
Molecular Weight:
110.04
Beilstein:
3568341
EC Number:
204-024-4
MDL number:
MFCD00002586
UNSPSC Code:
12352207
PubChem Substance ID:
24898615
NACRES:
NA.71

product line

BioReagent

Assay

≥99%

form

powder

technique(s)

cell culture | insect: suitable
cell culture | mammalian: suitable

mp

>300 °C (lit.)

solubility

H2O: 100 mg/mL

density

1.78 at 20 °C

application(s)

metabolomics

storage temp.

2-8°C

SMILES string

[Na+].CC(=O)C([O-])=O

InChI

1S/C3H4O3.Na/c1-2(4)3(5)6;/h1H3,(H,5,6);/q;+1/p-1

InChI key

DAEPDZWVDSPTHF-UHFFFAOYSA-M

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General description

Sodium pyruvate, derived from pyruvic acid, finds extensive use in cell biology, cell culture, and biochemical research. As the end-product of the glycolysis pathway, where glucose is converted into pyruvate with the energetic production of ATP (Adenosine Triphosphate), pyruvate serves as an intermediary in numerous metabolic pathways, including sugar metabolism. While cells naturally synthesize sodium pyruvate as an intermediate metabolite in glycolysis, it isn′t universally necessary as a supplement for all cell cultures. However, if cells have been cultivated in a medium enriched with sodium pyruvate, it′s advisable to continue its use as cell growth may diminish without it.Sodium pyruvate is frequently incorporated into cell culture formulations to fortify broths and media, enhancing cell survival by providing an easily-accessible carbohydrate energy source alongside glucose. It′s often added to the growth medium, particularly for rapidly proliferating cells like tumor cells. Furthermore, it participates in amino acid metabolism and initiates the Krebs cycle. Cells can readily utilize sodium pyruvate as a carbon source for energy production and crucial metabolic pathways, eliminating the need for biosynthetic production from glucose or amino acids.Certain cell lines require supplemental pyruvate in the culture media due to their inability to convert glucose or amino acids into pyruvate. Additionally, sodium pyruvate serves as a free radical scavenger, effectively quenching reactive oxygen species (ROS) and contributing to cellular health and viability.

Application

Sodium pyruvate has been used:
  • to culture MCF7 ρ0 (human breast cancer cell line) cells as parent cells
  • as a supplement in extracellular flux (XF) assay media to incubate cells for measuring oxygen consumption rate (OCR)
  • as a supplement in minimum essential medium Eagle to maintain breast cancer (MCF-7) cells
  • as a supplement in Dulbecco′s modified Eagle medium (DMEM)-based medium and modified Eagle medium (MEM)-α for testis cell culture to evaluate the effect of medium composition on in vitro SSC maintenance

Biochem/physiol Actions

Sodium Pyruvate has shown antioxidant properties and protective effects against oxygen radicals. Pyruvate is produced as part of glycolysis and is an intermediate in many metabolic pathways. It can be converted into acetyl CoA and enter the TCA Cycle.
Sodium pyruvate can be used as a component in culture broth and media. It exhibits protective effects in various animal models of ischemia-reperfusion injury, hemorrhagic shock, and septic shock when given exogenously.

Features and Benefits

  • Cell culture and insect cell culture tested

  • Versatile and adaptable for a wide variety of laboratory and research applications
  • BioReagent Grade Sodium succinate for your Cell Biology and Biochemical research
  • Tested for Mammalian and Insect cell culture applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

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Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H317,H319

Hazard Classifications

Eye Irrit. 2 - Skin Sens. 1B

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Rekin's Janky et al.
PLoS computational biology, 10(7), e1003731-e1003731 (2014-07-25)
Identifying master regulators of biological processes and mapping their downstream gene networks are key challenges in systems biology. We developed a computational method, called iRegulon, to reverse-engineer the transcriptional regulatory network underlying a co-expressed gene set using cis-regulatory sequence analysis.
Wei Yang et al.
Nature communications, 5, 3818-3818 (2014-05-03)
Polycomb Repressive Complex 1 and histone H2A ubiquitination (ubH2A) contribute to embryonic stem cell (ESC) pluripotency by repressing lineage-specific gene expression. However, whether active deubiquitination co-regulates ubH2A levels in ESCs and during differentiation is not known. Here we report that
Lotta Tengroth et al.
PloS one, 9(6), e98239-e98239 (2014-06-03)
The human nasal epithelium is an important physical barrier, and a part of the innate immune defense that protect against pathogens. The epithelial cells recognize microbial components by pattern-recognition receptors (PRRs), and thereby trigger an immune response. Even though TLR3
Philipp Müller et al.
Science translational medicine, 7(315), 315ra188-315ra188 (2015-11-27)
Targeted drug delivery with antibody-drug conjugates such as the HER2-directed ado-trastuzumab emtansine (T-DM1) has emerged as a powerful strategy for cancer therapy. We show that T-DM1 is particularly effective in eliciting antitumor immunity in patients with early breast cancer (WSG-ADAPT
Jonathan B Grimm et al.
Nature methods, 12(3), 244-250 (2015-01-20)
Specific labeling of biomolecules with bright fluorophores is the keystone of fluorescence microscopy. Genetically encoded self-labeling tag proteins can be coupled to synthetic dyes inside living cells, resulting in brighter reporters than fluorescent proteins. Intracellular labeling using these techniques requires
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