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Key Documents

P0425

Sigma-Aldrich

Poly-Prep Slides

poly-L-lysine coated glass slides

Synonym(s):

Poly-L-Lysine-coated glass slides

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About This Item

UNSPSC Code:
41116124
NACRES:
NA.47

Quality Level

200

shelf life

Expiry date on the label.

application(s)

hematology
histology

storage temp.

room temp

General description

Poly-Prep slides are made of glass and designed for cell adhesion. The slides are coated with Poly-L-Lysine, a non-specific attachment factor for cells useful in promoting cell adhesion on solid substrates. Poly-L-lysine enhances binding between the cell membrane and the cultured surface.

Application

Poly-Prep Slides are used:
  • To mount liver and lung cell sections and mounted on poly-prep slides.
  • To prove better cell adhesion in clinical bronchoalveolar lavage fluid samples.
  • To determine pAkt (alpha serine/threonine-protein kinase) expression by immunohistochemistry.
  • In immunofluorescence imaging of nuclear translocation of nuclear factor κB(NFκB) in A549 cells.
  • To study the phospholipase C-zeta activity of sperm.
  • To prepare coelomocytes smears, which were further subjected to antibody staining.
Overall, glass slides coated with poly-L-lysine for tissue section adhesion in routine histological uses. And, it is not recommended for cell culture or microarray applications.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Expression pattern of arenicins?the antimicrobial peptides of polychaete Arenicola marina
Maltseva AL, et al.
Frontiers in Physiology, 5, 497-497 (2014)
Yu Zheng et al.
Nature communications, 8, 14344-14344 (2017-02-10)
Metastasis-competent circulating tumour cells (CTCs) experience oxidative stress in the bloodstream, but their survival mechanisms are not well defined. Here, comparing single-cell RNA-Seq profiles of CTCs from breast, prostate and lung cancers, we observe consistent induction of β-globin (HBB), but
The effect of prolonged incubation and temperature on oocyte activator phospholipase C-zeta activity of sperm
Kervancioglu DE, et al.
Marmara medical journal, 629-629 (2017)
Clinical significance of Akt and HER2/neu overexpression in African-American and Latina women with breast cancer
Wu Y, et al.
Breast Cancer Research, 10(1), R3-R3 (2008)
Amhed M Vargas-Velazquez et al.
Genetics, 211(4), 1315-1330 (2019-02-01)
Genetic screens in the nematode Caenorhabditis elegans identified the EGF/Ras and Notch pathways as central for vulval precursor cell fate patterning. Schematically, the anchor cell secretes EGF, inducing the P6.p cell to a primary (1°) vulval fate; P6.p in turn
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