N5632

Nile Blue A

Dye content ≥75 %

• Synonym(s): Basic Blue 12, Nile blue sulfate
• Linear Formula: 2C₂₀H₂₀N₃O · SO₄
• CAS Number: 3625-57-8
• Molecular Weight: 732.85
• Colour Index Number: 51180
• EC Number: 222-832-5
• MDL number: MFCD00064529
• UNSPSC Code: 12171500
• PubChem Substance ID: 24897743
• NACRES: NA.47

Properties

• form: powder
• Quality Level: 100
• composition: Dye content, ≥75%
• mp: >300 °C (dec.) (lit.)
• solubility: H₂O: 1 mg/mL, blue
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [O-]S([O-])(=O)=O.CC\[N+](CC)=C1/C=CC2=Nc3c(OC2=C1)cc(N)c4ccccc34.CC\[N+](CC)=C5/C=CC6=Nc7c(OC6=C5)cc(N)c8ccccc78
• InChI: 1S/2C20H19N3O.H2O4S/c2*1-3-23(4-2)13-9-10-17-18(11-13)24-19-12-16(21)14-7-5-6-8-15(14)20(19)22-17;1-5(2,3)4/h2*5-12,21H,3-4H2,1-2H3;(H2,1,2,3,4)
• InChI key: QIRDPEPUXNCOLD-UHFFFAOYSA-N

Description

General description

Nile Blue A is aminodiethylaminonaphthophenoxazonium sulfate employed in biology and histology. It is a highly fluorescent, and photostable organic dye used to dye clothing, and is for use in lipid histochemistry.

Application

Nile blue A is suitable for staining and discriminating between melanins and lipofuscins in paraffin sections of animal tissue, and in a histochemical method for lipids in frozen sections.

Biochem/physiol Actions

Nile blue A stains acidic components, such as phospholipids, nucleic and fatty acids by imparting a dark blue color, while neutral lipids, namely triglycerides, are stained pink, or red.

Features and Benefits

Used in the preparation of an amperometric glucose sensor.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves