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L8757

Sigma-Aldrich

Lysing Enzymes from Rhizoctonia solani

crude powder

Synonym(s):

Kitalase

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About This Item

UNSPSC Code:
12352204

form

crude powder

storage temp.

2-8°C

Application

Lysing enzymes at a concentration of 1mg/ml can be used for preparation of protoplasts from Rhizoctonia solani.

Biochem/physiol Actions

Main enzymatic acitivity is β−(1-3)-glucanase, also reported to contain protease, pectinase, and amylase activities.

Certificates of Analysis (COA)

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Expression of a cephalosporin C esterase gene in Acremonium chrysogenum for the direct production of deacetylcephalosporin C
Basch, J., et al
Journal of Industrial Microbiology, 31(11), 531-539 (2004)
Structural characterization of a neutral antitumour β-D-glucan extracted with hot sodium hydroxide from cultured fruit bodies of Grifola frondosa.
Iino, K., et al
Carbohydrate Research, 141(1), 111-119 (1985)
Molecular characterization of a Stagonospora nodorum lipase gene LIP1
Feng, J., et al
Plant Pathology, 60(4), 698-708 (2011)
Tung-Hsen Liu et al.
New biotechnology, 27(1), 64-69 (2009-09-01)
Novozym 234 was the most frequently used enzyme for production of Rhizoctonia solani protoplasts. Since manufacture of this enzyme was discontinued in the late 1990s, a new procedure was developed by testing lytic enzymes from Sigma and by examining factors
D Tsuchiya et al.
Phytopathology, 91(4), 354-360 (2008-10-24)
ABSTRACT Cytological karyotypes with mitotic metaphase chromosomes were analyzed for Cochliobolus heterostrophus, C. carbonum, and C. sativus by the germ tube burst method (GTBM). Prior to karyotyping, procedures of GTBM suitable to Cochliobolus were established by examining several crucial conditions

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