I3036

Polyinosinic–polycytidylic acid potassium salt

γ-irradiated

• Synonym(s): Poly (I:C), Poly(I) • Poly(C)
• CAS Number: 31852-29-6
• MDL number: MFCD00131983
• UNSPSC Code: 41106305
• NACRES: NA.51

Properties

• Assay: ≥99% (TLC)
• composition: , 8-12% UV
• storage temp.: −20°C

Description

Biochem/physiol Actions

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

Packaging

Package size based on polynucleotide content

Other Notes

Double-stranded homopolymer.

Physical form

10% Poly (I:C) with sodium chloride and sodium phosphate buffer salts

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable