H2126

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

• Synonym(s): Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• CAS Number: 37326-33-3
• Enzyme Commission number: 3.2.1.35 (BRENDA, IUBMB)
• EC Number: 253-464-3
• MDL number: MFCD00131351
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• conjugate: conjugate (Glucosaminoglycan)
• type: Type II
• form: lyophilized powder
• specific activity: ≥300 units/mg solid
• mol wt: 55 kDa
• storage temp.: −20°C

Description

General description

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Application

Hyaluronidase from sheep testes has been used:

• in flow cytometry
• in the isolation of putative spermatogonial stem cells (SSCs)
• to study the effects of magnetic stimulation in magnetic hydrogel properties
• in immunohistochemistry to visualize collagen type II distribution
• to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
• for the degeneration of hydrogels

Biochem/physiol Actions

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Unit Definition

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Physical form

Lyophilized powder containing lactose

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves