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Key Documents

G6148

Sigma-Aldrich

Glasgow Minimum Essential Medium

With L-glutamine, without tryptose phosphate broth and sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

BHK medium, GMEM

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About This Item

MDL number:
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: 4.5 g/L
phenol red: 0.0213 g/L
L-glutamine: 0.292 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

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General description

Glasgow Minimum Essential Medium is suitable for the study of genetic factors affecting cell competence.

Application

Glasgow Minimum Essential Medium has been used:
  • in toxin entry assays
  • for 3 H-palmitate labeling
  • to culture embryonic stem cells

Quantity

Formulated to contain 12.5 grams of powder per liter of medium.

Reconstitution

Supplement with 2.75 g/L sodium bicarbonate and Tryptose phosphate broth solution (T 8159) at 100 ml/L of medium.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Eye Dam. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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