F5883
4-Fluoro-7-nitrobenzofurazan
≥98% (elemental analysis)
Synonym(s):
NBD-F
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About This Item
Empirical Formula (Hill Notation):
C6H2FN3O3
CAS Number:
Molecular Weight:
183.10
Beilstein:
1077571
MDL number:
UNSPSC Code:
12352100
PubChem Substance ID:
NACRES:
NA.32
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Quality Level
Assay
≥98% (elemental analysis)
mp
52-54 °C (lit.)
fluorescence
λex 380 nm; λem 515 nm (hydrolyzed (esterase))
storage temp.
2-8°C
SMILES string
[O-][N+](=O)c1ccc(F)c2nonc12
InChI
1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H
InChI key
PGZIDERTDJHJFY-UHFFFAOYSA-N
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Related Categories
Application
Useful for fluorescent labeling of amino acids and amines in HPLC
Packaging
Bottomless glass bottle. Contents are inside inserted fused cone.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
235.4 °F - closed cup
Flash Point(C)
113 °C - closed cup
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Bryan R Fonslow et al.
Analytical chemistry, 80(9), 3182-3189 (2008-03-21)
The continuous nature of micro free-flow electrophoresis (mu-FFE) was used to monitor the effect of a gradient of buffer conditions on the separation. This unique application has great potential for fast optimization of separation conditions and estimation of equilibrium constants.
Chanda Ciriacks Klinker et al.
Analytical chemistry, 79(22), 8747-8754 (2007-10-13)
4-Fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) has been characterized as a fluorogenic labeling reagent for high speed, online microdialysis-capillary electrophoresis (MD-CE) assays for amino acid neurotransmitters. NBD-F labeled amines are efficiently excited using 488 nm light allowing more common lasers to be used for
Zsolt Wagner et al.
Electrophoresis, 32(20), 2816-2822 (2011-10-20)
Three different fluorescent tags have been compared for the quantitative analysis of aspartate and glutamate in brain microdialysate samples. Separation conditions have been optimized to achieve short analysis time using reversed polarity separation in coated capillary. Method validation has revealed
Hua-Ming Tseng et al.
Analytical and bioanalytical chemistry, 388(2), 433-439 (2007-03-21)
A rapid and sensitive capillary electrophoresis (CE) method has been developed for profiling organic metabolites containing amine functional groups in mammalian biofluids. Metabolites containing an amine group were derivatized with 4-fluoro-7-nitrobenzo-2,1,3-oxadiazol (NBD-F), separated by micellar electrokinetic chromatography (MEKC), and detected
Yanting Song et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(5-6), 335-340 (2011-01-19)
A fast HPLC method using a monolithic silica column was developed for the measurement of amino acids. The amino acids were pre-column derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and separated on a monolithic silica column (MonoClad C18-HS, 250 mm × 3 mm
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