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D4293

Sigma-Aldrich

3,3′-Diaminobenzidine

SIGMAFAST™, chromogenic, tablet

Synonym(s):

DAB tablets

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About This Item

CAS Number:
91-95-2
MDL number:
MFCD00007725
UNSPSC Code:
12352204
PubChem Substance ID:
24893856
NACRES:
NA.83

product name

SIGMAFAST™ 3,3′-Diaminobenzidine tablets, tablet, To prepare 5 mL

form

tablet

storage temp.

−20°C

SMILES string

Nc1ccc(cc1N)-c2ccc(N)c(N)c2

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

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Application

SIGMAFAST™ 3,3′-Diaminobenzidine tablets has been used as a chromogen for visualization in immunohistochemistry of gonad tissue, thymus, dental chronic periapical lesions and gall bladder sections.
Developed for use in immunohistology and immunoblotting as a precipitating substrate for the localization of peroxidase activity. DAB is the immunohistology substrate of choice as it produces an intense brown-black stain which is resistant to alcohol. Slides stained with DAB may be dehydrated, mounted in resinous media, and stored for future reference. SIGMAFAST™ DAB Tablets require no additional buffers or steps to prepare an active substrate solution. Tablets are individually packaged in foil packets

Reconstitution

Each tablet set dissolved in 5 mL deionized water yields a ready-to-use buffered solution containing DAB and urea hydrogen peroxide.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Legal Information

SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Signal Word

Danger

Hazard Statements

H315,H318,H335,H341,H350

Hazard Classifications

Carc. 1B - Eye Dam. 1 - Muta. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Receptor activator NFkappaB-ligand and osteoprotegerin protein expression in human periapical cysts and granulomas
Menezes R, et al.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontics, 102(3), 404-409 (2006)
Expression of pluripotent stem cell markers in the human fetal testis
Kerr CL, et al.
Stem Cells, 26(2), 412-421 (2008)
Interstitial cells of Cajal are present in human extrahepatic bile ducts
Ahmadi O, et al.
Journal of Gastroenterology and Hepatology, 25(2), 277-285 (2010)
Cristian Tomasetti et al.
Proceedings of the National Academy of Sciences of the United States of America, 116(41), 20482-20488 (2019-09-25)
A new evaluation of previously published data suggested to us that the accumulation of mutations might slow, rather than increase, as individuals age. To explain this unexpected finding, we hypothesized that normal stem cell division rates might decrease as we
Increased Bax/Bcl-2 ratio up-regulates caspase-3 and increases apoptosis in the thymus of patients with myasthenia gravis
Salakou S, et al.
In Vivo, 21(1), 123-132 (2007)
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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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