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biological source
human colon
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
Elevated levels of cystic fibrosis transmembrane conductance regulator (CFTR) protein
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human Caucasian colon adenocarcinoma
Cell Line Description
A subclone of the parent HT29-18 differentiated to specific cell types for studies on cystic fibrosis.
Culture Medium
DMEM + 2mM Glutamine + 1mM Sodium Pyruvate (NaP) + 44mM Sodium Bicarbonate (NaHCO3) + 10mg/l Transferrin + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 to 1:8, i.e. seeding at 1-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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