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Key Documents

OX-40

Reactivity: Anti-CD134, rat, 86100906

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About This Item

UNSPSC Code:
41106514

biological source

Mouse x Mouse Hybridoma

ImmunoDon

BALB/c mouse spleen

Myeloma

NS1/1

Reactivity

Anti-CD134, rat

technique(s)

cell culture | mammalian: suitable

isotype

IgG2b

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Mouse NS1/1 x Mouse BALB/c spleen

Cell Line Description

The antibody recognises an antigen detected only on rat T blasts that also express the CD4 antigen. The OX-40 antigen is a distinct chain of molecular weight 45-50kDa. OX-40 monoclonal antibody augments T cell proliferation at late stages of in vitro responses.

Immunogen

PHA-activated rat lymph node cells

Culture Medium

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Subculture Routine

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Disclaimer

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

Certificates of Analysis (COA)

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