RK-13 Cell Line from rabbit

21715, rabbit kidney, Epithelial-like

• Synonym(s): GL RK/13 Cells, Glaxo Laboratories Rabbit Kidney 13 Cells, R13 Cells
• UNSPSC Code: 41106514
• eCl@ss: 32190102

Properties

• Product Name: RK-13 Cell Line from rabbit, rabbit kidney, 00021715
• biological source: rabbit kidney
• growth mode: Adherent
• karyotype: 2n=44, stem-line = 56 (=22%)
• morphology: Epithelial-like
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Rabbit kidney

Cell Line Description

RK13 has been derived from kidney cells of a 5 week old rabbit. The cell line has been shown to be susceptible to pseudorabies virus, rabbitpox virus, myxomatose virus, vaccinia and EAV. Sensitivity to other viruses such as Simian adenovirus and rubella has been reported as well with the production of cytopathic effect (CPE). RK13 has been successfully treated with thiamutin for eradication of mycoplasma. The Bovine Viral Diarrhoea Virus (BVDV) status of this cell line was previously described as negative. Results of recent multiple molecular tests for BVDV that were undertaken by two independent testing laboratories were equivocal. This means that we cannot confirm the BVDV status of this cell line.

Application

Virus studies

Culture Medium

EMEM(HBSS) (M5775) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:4 using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37ºC; monolayer is confluent after 48-56 hrs and stable for at least 21d; small syncytia in elder cultures are typical.

Other Notes

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