C0897

Citrate Lyase from Klebsiella pneumoniae

lyophilized powder, ≥0.20 unit/mg solid

• Synonym(s): Citrate oxaloacetate lyase
• CAS Number: 9012-83-3
• Enzyme Commission number: 4.1.3.6 (BRENDA, IUBMB)
• EC Number: 232-740-7
• MDL number: MFCD00130807
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: lyophilized powder
• Quality Level: 200
• specific activity: ≥0.20 unit/mg solid
• mol wt: >5 kDa
• storage temp.: 2-8°C

Description

Application

Citrate Lyase from Klebsiella pneumoniae has been used in the digestion of low molecular weight human milk fraction (>5 kDa referred to as 5kF).

Biochem/physiol Actions

Citrate lyase is found in several microorganisms and catalyzes the first step of citrate degradation, forming acetate and oxaloacetate. The enzyme contains 3 polypeptide subunits, α-subunit (a transferase), β-subunit (acyl lyase) and γ-subunit (acyl-carrier protein).

Unit Definition

One unit will convert 1.0 μmole of citrate to oxalacetate per min at pH 7.6 at 25 °C.

Physical form

Lyophilized powder containing bovine serum albumin, sucrose, and MgSO₄

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves