PPB005
Phosphate Buffered Saline with 0.05% TWEEN® 20, pH 7.4
pHast Pack™, powder
Synonym(s):
PBS + 0.05% TWEEN® 20, PBS with 0.05% Tween 20, PBS-T, PBST
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About This Item
UNSPSC Code:
12161700
NACRES:
NA.21
Recommended Products
product line
pHast Pack™
form
powder
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General description
Phosphate Buffered Saline with 0.05% Tween® 20 or PBST contains PBS buffer with a low-concentration of Tween® detergent. It is a common wash buffer and due to the presence of detergent, it can easily remove or block excess components thus reducing foaming and non-specific binding on the membrane and microtiter plate wells.
Application
PBST is used in research in a variety of assays including DNA or RNA hybridization assays and cell culture. It is commonly used for immunoassays like Western blot, ELISA, Dot blot, Immunohistochemistry (IHC), immunopercipitation (IP), and immunofluorecence. It has been used for incubation and wash buffer for peptide microarrays for performing High-density peptide array (HDPA) to analyze antibody responses against SARS-CoV-2.
Features and Benefits
- Ready-to-use buffer
- No measuring and pH adjusting needed
- Save time and minimize effort
- Biological tests: free of DNase, RNase, protease, and nickase
- Chemical tests: Iron ≤10 ppm, lead ≤5 ppm
Packaging
Foil pouches
Reconstitution
Contents of one pouch, when dissolved in 500 mL of distilled or deionized water, will yield a 1× solution containing 10 mM Na2HPO4, 1.8 mM KH2PO4, 138 mM NaCl, 2.7 mM KCl; 0.05% Tween 20 (w/v), pH 7.4 at 25 °C. Certified to be DNAse, RNAse, Protease, and Nickase free.
Storage and Stability
Store at room temperature. Product may naturally agglomerate but can be simply broken up within the pouch prior to use.
Other Notes
For additional information on our range of Biochemicals, please complete this form.
Legal Information
TWEEN is a registered trademark of Croda International PLC
pHast Pack is a trademark of Sigma-Aldrich Co. LLC
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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