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55420

Millipore

CLED Agar

suitable for microbiology, NutriSelect® Plus

Synonym(s):

BROLACIN Agar, Bromothymol-blue Lactose Cystine Agar, Cystine-Lactose-Electrolyte Deficient Agar

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15 g/L
beef extract, 3 g/L
bromo thymol blue, 0.02 g/L
casein enzymic hydrolysate, 4 g/L
L-cystine, 0.128 g/L
lactose, 10 g/L
peptic digest of animal tissue, 4 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | utilization test: suitable
microbiological culture: suitable

final pH

7.3±0.2 (25 °C)

application(s)

clinical testing
environmental
food and beverages
microbiology

suitability

nonselective and differential for Enterococcus spp.
nonselective and differential for Escherichia coli
nonselective and differential for Klebsiella spp.
nonselective and differential for Lactobacillus spp.
nonselective and differential for Proteus spp.
nonselective and differential for Pseudomonas spp.
nonselective and differential for Salmonella spp.
nonselective and differential for Staphylococcus spp.
nonselective and differential for Streptococcus spp.
nonselective and differential for coliforms

General description

CLED (cysteine-, lactose- and electrolyte-deficient) Agar is recommended for isolation, enumeration and identification of urinary pathogens.
CLED Agar is commonly used in bacteriological analysis.

Application

CLED Agar has been used as a non-selective media to perform enantiomeric profiling of quinolones and quinolones resistance gene qnrS in wastewater samples

Preparation Note

Suspend 36.15g in 1000ml distilled water. Boil to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG6199
BCCF6789
BCCF1310
BCCC4353
BCCB7076

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B Bogokowsky et al.
The International journal of applied radiation and isotopes, 34(10), 1441-1443 (1983-10-01)
MacConkey agar and Cystine-Lactose-Electrolyte-Deficient (CLED) agar, media widely used in the bacteriological laboratory and recommended for the detection of urinary tract infections, were sterilized by gamma-radiation at a dose of 1.5 Mrad. Both were modified and adapted to radiation sterilization
Erika Castrignanò et al.
Water research, 175, 115653-115653 (2020-03-26)
Wastewater-based epidemiology (WBE) was applied for the first time in seven cities across Europe with the aim of estimating quinolones consumption via the analysis of human urinary metabolites in wastewater. This report is also the first pan-European study focussed on

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