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Millipore

Rapid Coliform ChromoSelect Broth

suitable for microbiology, NutriSelect® Plus

Synonym(s):

Coliform Rapid ChromoSelect Broth

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

200

form

powder

shelf life

limited shelf life, expiry date on the label

composition

chromogenic substrate, 0.08 g/L
dipotassium hydrogen phosphate, 2.7 g/L
fluorogenic substrate, 0.05 g/L
IPTG, 0.1 g/L
peptone, special, 5 g/L
potassium dihydrogen phosphate, 2 g/L
sodium chloride, 5 g/L
sodium lauryl sulfate, 0.1 g/L
sorbitol, 1 g/L

manufacturer/tradename

NutriSelect® Plus

final pH

6.8±0.3 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
water monitoring
microbiology

storage temp.

2-8°C

suitability

selective and differential for Escherichia coli
selective and differential for coliforms

Application

Rapid Coliform ChromoSelect Broth is used for detection and conformation of Escherichia coli and coliforms on the basics of enzyme substrate reaction from water samples, using a combination of chromogenic and fluorogenic substrate.

Preparation Note

Suspend 16.0 g (single strength) or 32.0 g (double strength) in 1 litre distilled water. Boil to dissolve the medium completely. Dispense as desired and sterilize by autoclaving at 1 bar pressure (121 °C) for 15 minutes.

Other Notes

Medium for the assay of water for Coliforms

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCD6203
BCBT8396
BCBS2398V
BCBG5660V
BCBG5660

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G. Hahn, E. Wittrock
Acta Microbiologica Academiae Scientiarum Hungaricae, 38, 225-225 (1991)
M Manafi et al.
Microbiological reviews, 55(3), 335-348 (1991-09-01)
Methods based on the application of chromogenic and fluorogenic substrates enable specific and rapid detection of a variety of bacterial enzymatic activities. By using these techniques, enzymatic reactions can be examined simultaneously or individually, either directly on the isolation plate

Articles

Overview of Chromogenic Media

Selective media enable faster results and visual confirmation for the detection, identification, and enumeration of microorganisms

Selective Growth Media for Differentiation and Detection of Escherichia coli and Other Coliforms

An article concerning selective growth media for differentiation and detection of Escherichia coli and other coliforms.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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