114367

Bromocresol Green sodium salt

90% (HPLC), ACS reagent, Dye content 90 %

• Synonym(s): 3′,3″,5′,5″-Tetrabromo-m-cresolsulfonphthalein sodium salt
• Empirical Formula (Hill Notation): C₂₁H₁₃Br₄NaO₅S
• CAS Number: 62625-32-5
• Molecular Weight: 720.00
• EC Number: 263-657-4
• MDL number: MFCD00148898
• UNSPSC Code: 12171500
• PubChem Substance ID: 24847167

Properties

• grade: ACS reagent
• Quality Level: 200
• Agency: suitable for SM 2310; suitable for SM 2320
• Assay: 90% (HPLC)
• form: powder or crystals
• composition: Dye content, 90%
• visual transition interval: 3.8, yellow(Visual Interval Pass); 3.8-5.2, yellow to blue; 4.5, green; 5.4, blue(Visual Interval Pass)
• mp: 230 °C (dec.) (lit.)
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].Cc1c(Br)c(O)c(Br)cc1\C(=C2\C=C(Br)C(=O)C(Br)=C2C)c3ccccc3S([O-])(=O)=O
• InChI: 1S/C21H14Br4O5S.Na/c1-9-12(7-14(22)19(26)17(9)24)21(13-8-15(23)20(27)18(25)10(13)2)11-5-3-4-6-16(11)31(28,29)30-21;/h3-8,26-27H,1-2H3;/q;+1/p-1
• InChI key: HEFSGAHJDGZCHA-UHFFFAOYSA-M

Description

General description

Bromocresol Green (BCG), also known as bromcresol green, is a synthetic, anionic, triphenylmethane dye belonging to the phthalein and sulphonphthalein dyes group. It is commonly used as a pH indicator as it changes color from yellow to blue-green at pH 3.8-5.4. The monosodium salt of bromocresol green is highly ionizable in water. The dye at a higher pH level produces a large and weakly lipophilic dianion.

Application

Bromocresol green serves as a pH indicator in biology and medicinal experiments.

• It is used widely as a vital stain to study blood-brain permeability.
• It is used as a tracking dye for DNA agarose gel electrophoresis, in protein determinations, and in charge-transfer complexation processes.
• It is used in TLC for the visualization of compounds with functional groups whose pKa is below 5.0.
• It has been used in the pH-sensitive colorimetric determination of glutamate decarboxylase activity.
• It has also been employed in the evaluation of paper-analytical devices for colorimetric analysis of serum samples.
• It is suitable as a selective inhibitor in kidney physiology studies.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves