SFLCKB
Roche
KAPA SYBR® FAST
suitable for qPCR, 2 ×, for LightCycler®480
Synonym(s):
Lightcycler, qPCR
About This Item
Quality Level
usage
sufficient for 100 reactions
sufficient for 1000 reactions
sufficient for 500 reactions
shelf life
≤12 mo.
feature
dNTPs included
hotstart
packaging
kit of 1 mL (100 x 20 μL rxn; KK4609)
kit of 10 mL (1000 x 20 μL rxn; KK4611)
kit of 5 mL (500 x 20 μL rxn; KK4610)
manufacturer/tradename
Roche
concentration
2 ×
technique(s)
qPCR: suitable
input
purified DNA
compatibility
for use with Bio-Rad CFX96
for use with Bio-Rad Chromo4 (Real-Time Detector)
for use with Bio-Rad DNA Engine Opticon
for use with Bio-Rad Opticon 2
for use with Eppendorf® Mastercycler (ep realplex )
for use with Illumina Eco
for use with Roche LightCycler 1.5/2.0
for use with Roche LightCycler 480
for use with Roche LightCycler 96
for use with Roche LightCycler Nano
for use with Rotor-Gene®
for use with Smart Cycler®
detection method
SYBR® Green
storage temp.
−20°C
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General description
The improved robustness, processivity, and speed of KAPA SYBR FAST qPCR Kits result in consistently high amplification efficiencies enabling more accurate relative quantification for gene expression analysis. KAPA SYBR FAST qPCR Kits, developed to perform optimally in stringent real-time PCR reaction conditions, exhibit dramatic improvements to signal-to-noise ratio (fluorescence), quantification cycle (Cq), linearity, and sensitivity.
Application
- Gene expression
- Gene knockdown validation
- Microarray validation
- ChIP analysis
- Low copy detection
- Absolute quantification of NGS libraries
- Quantitative real-time PCR (qPCR)
Features and Benefits
- High reaction efficiency between 95 - 105% improves accuracy and reproducibility.
- Unbiased efficiency across a wide range of GC contents and amplicon lengths.
Detect low copy and difficult targets consistently
- Improved processivity results in earlier Cq scores.
- Higher fluorescence detection across varying AT- and GC-rich targets.
- Novel enzyme is resistant to SYBR® inhibition.
Complete real-time PCR runs in just 40 minutes
- 55% shorter run times with fast cycling protocol.
- Maintain high performance when switching from standard to fast protocols.
Quick Notes:
- This kit contains an engineered enzyme optimized for qPCR using SYBR Green I dye chemistry.
- The 2X master mix contains a proprietary buffer. Together with the novel enzyme, this improves amplification efficiency of both GC- and AT-rich targets.
- 20 sec initial denaturation at 95°C is sufficient for enzyme activation. When working with complex templates, an initial denaturation of 3 min is recommended.
- For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition at 72°C.
- Do not exceed 25 μL reaction volumes.
Quality
Preparation Note
The SYBR Green I dye contained in KAPA SYBR FAST qPCR Master Mix (2X) and ROX/fluorescein dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity.
KAPA SYBR FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week and may be stored at this temperature for short-term use, provided that they do not become contaminated with microbes and/or nucleases.
Other Notes
Legal Information
Kit Components Only
- KAPA SYBR® FAST DNA Polymerase
- reaction buffer
- dNTPs
- SYBR® Green I dye
- MgCl2 2.5 mM
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
STOT SE 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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