11534378910
Roche
Tetramethyl-Rhodamine-5-dUTP
Synonym(s):
Rhodamine-5-dUTP tetralithium salt, Tetramethylrhodamine-5(6)-(5-[3-carboxamidoallyl]-2′-deoxyuridine 5′-triphosphate)
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About This Item
Recommended Products
grade
Molecular Biology
Quality Level
Assay
97.7% (HPLC)
form
solution
mol wt
990.5
packaging
pkg of 25 μL (25 nmol; 1mM)
manufacturer/tradename
Roche
concentration
1.0 mmol/L (Tetramethylrhodamine-5-dUTP (Abs. 551 nm))
color
clear red
mp
~0 °C
solubility
water: miscible
storage temp.
−20°C
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General description
Tetramethylrhodamine is bound to deoxyuridine triphosphate via an amide linkage.
Application
Tetramethyl-Rhodamine-5-dUTP has been used in the labelling of DNA fragments and in the synthesis of fluorescence in situ hybridization (FISH) probes.
Quality
Typical analysis: At least 85% Tetramethyl-Rhodamine-5-dUTP (HPLC, area%).
Analysis Note
Absorption: Absorption spectra available in the files.
Other Notes
For life science research only. Not for use in diagnostic procedures. For some in situ experiments, it is useful to label DNA with two fluorescent "tags," tetramethylrhodamine (red) and fluorescein (yellow).
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
does not flash
Flash Point(C)
does not flash
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