MT17MAG47K-PX25

MILLIPLEX^® Mouse TH17 Magnetic Bead Panel - Premixed 25 plex - Immunology Multiplex Assay

Simultaneously analyze multiple Th17 cytokine and chemokine biomarkers with the Th17 Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

• UNSPSC Code: 12161503
• eCl@ss: 32161000

Properties

• Quality Level: 200
• species reactivity: mouse
• manufacturer/tradename: Milliplex^®
• assay range: accuracy: 85-110%; linearity: 111.0%; (1:04); linearity: 113.1%; (1:08); standard curve range: 1.5-1,500 pg/mL; (IL-4); standard curve range: 10-10,000 pg/mL; (IL-17F); standard curve range: 127-130,000 pg/mL; (IL-28B); standard curve range: 15-15,000 pg/mL; (IL-1β); standard curve range: 2.4-2,500 pg/mL; (IL-22); standard curve range: 20-20,000 pg/mL; (IL-10, IL-12(p70), & IL-21); standard curve range: 3.4-3,500 pg/mL; (TNFα); standard curve range: 34-35,000 pg/mL; (GM-CSF & IL-15); standard curve range: 342-350,000 pg/mL; (IL-23); standard curve range: 39-40,000 pg/mL; (IL-13 & IL-17A); standard curve range: 4.9-5,000 pg/mL; (IL-5); standard curve range: 488-500,000 pg/mL; (TNFβ); standard curve range: 49-50,000 pg/mL; (CD40L IL-31 & MIP-3α); standard curve range: 586-600,000 pg/mL; (IL-17E/ IL-25); standard curve range: 6.9-6,000 pg/mL; (IL-2); standard curve range: 7.8-8,000 pg/mL; (IFNγ & IL-6); standard curve range: 78-80,000 pg/mL; (IL-33); standard curve range: 879-900,000 pg/mL; (IL-27)
• technique(s): multiplexing: suitable
• compatibility: configured for Premixed
• detection method: fluorometric (Luminex xMAP)
• shipped in: wet ice

Description

General description

CD4 T-helper cells are major players in adaptive immunity. Based on their expression profile of transcription factors and secreted cytokines, these cells are divided into the major subsets Th1, Th2, Th17 and T regulatory (Treg) cells. Th1 cells, induced by IL-12 and enhanced by IFNγ, secrete IFNγ that activates macrophages. Induced by IL-4, Th2 cells primarily produce IL-4, IL-5, IL-13, IL-25 and IL-17E, playing a role in allergy. Treg cells, induced by TGFβ, produce the anti-inflammatory cytokines IL-10 and TGFβ; and function to control T-cell responses to prevent autoimmune reactions. Th17 cells, a more recently discovered subset characterized by the production of IL-17, are induced by TGFβ; combined with IL-23 and IL-1β; in humans, and TGFβ, IL-6 and IL-21 in mice. Activated Th17 cells secrete IL-17A, IL-17F, IL-21, IL-22, and TNFα; to promote tissue inflammation.

Th17 cells are involved in the clearance of extracellular bacteria and fungi. They are abundant in the intestinal lamina propria and function as a barrier against invading pathogens. Excessive amounts of Th17 cells have been implicated in the pathogenesis of several autoimmune diseases, including multiple sclerosis, psoriasis, juvenile diabetes, rheumatoid arthritis, Crohn′s disease, and autoimmune uveitis. In addition, Th17 cells play an important role in tumor development, progression, and metastasis, potentially in both promoting and inhibiting tumor growth.

The MILLIPLEX^® Mouse Th17 Bead panel enables you to focus on the therapeutic potential of the modulation of CD4 T-helper cell cytokines. Coupled with the Luminex^® xMAP^®platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.

Panel Type: Cytokines/Chemokines

Specificity

Cross Reactivty
There was no or minimum cross-reactivity between the antibodies and any other analytes in the panel (see details in assay protocol).

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Application

• Analytes: CD40 Ligand, GM-CSF, IFN-γ, IL-1β, IL-2, IL-4, IL-5, IL-6, IL-10, IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-21, IL-22, IL-23, IL-27, IL-28B, IL-31, IL-33, MIP-3α/CCL20, TNF-α, TNFβ
• Recommended Sample type: serum, plasma or tissue/cell lysate and culture supernatant
• Recommended Sample dilution: neat
• Assay Run Time: overnight or one day
• Research Category: Inflammation & Immunology

Storage and Stability

Recommended storage for kit components is 2 - 8°C.

Other Notes

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Pictograms: GHS05,GHS07,GHS08,GHS09
• Signal Word: Danger
• Hazard Statements: H302 + H312 + H332 - H317 - H318 - H373 - H411
• Precautionary Statements: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Hazard Classifications: Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Target Organs: Respiratory Tract
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3