MAB342
Anti-Galactocerebroside Antibody, clone mGalC
clone mGalC, Chemicon®, from mouse
Synonym(s):
Galactoceramide, Galactocerebroside, Galactosylceramide, GalC
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
biological source
mouse
Quality Level
antibody form
purified antibody
clone
mGalC, monoclonal
species reactivity
mouse, human, rabbit, bovine, chicken, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
isotype
IgG3
shipped in
dry ice
target post-translational modification
unmodified
General description
Galactocerebroside (GalC) is a major galactosphingolipid of myelin which plays a role in myelination. GalC is a very useful, specific marker for oligodendroglial lineage.
Specificity
Galactocerebroside (GalC), sulfatide, psychosine and other galactolipids. Cross-reacts with the sulfatide ester of GalC, but to a 16-fold lesser extent. No cross-reactivity with sphingosine, ceramide, mixed ganglioside or glucocerebroside. Binds specifically with oligodendrocytes and Schwann cells.
Immunogen
Synaptic plasma membranes from bovine hippocampus. Ranscht, B. et al. PNAS 79(8):2709-2713 (1982)
Application
Anti-Galactocerebroside Antibody, clone mGalC is an antibody against Galactocerebroside for use in ELISA, IC, & IHC.
Immunohistochemistry:
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.
Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.
ELISA:
A previous lot was used on purified galactocerebrosides.
Optimal working dilutions must be determined by the end user.
0.5-10 μg/mL of a previous lot was used on formalin fixed frozen sections of a prevous lot. Can not be used on paraffin embedded tissue sections since the antigen is denatured during embedding and paraffin removal.
Immunocytochemistry:
0.5-10 μg/mL of a previous lot was used on 4% paraformaldehyde, acetic acid or ethanol fixed cultured cells.
ELISA:
A previous lot was used on purified galactocerebrosides.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neuronal & Glial Markers
Neuronal & Glial Markers
Physical form
Format: Purified
Protein A purified
Purified mouse monoclonal in buffer containing 10 mM Potassium Phosphate, 150 mM NaCl, pH 7.4 containing 0.09% sodium azide.
Storage and Stability
Stable for 1 year at -20ºC from date of receipt.
Analysis Note
Control
Neonatal cortex
Neonatal cortex
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Oligodendrocyte dysfunction after induction of experimental anterior optic nerve ischemia.
Goldenberg-Cohen, N; Guo, Y; Margolis, F; Cohen, Y; Miller, NR; Bernstein, SL
Investigative Ophthalmology & Visual Science null
T Matsushita et al.
Neuroscience, 136(1), 115-121 (2005-09-27)
The successive stages of development from oligodendrocyte progenitor to mature oligodendrocyte have been investigated in detail by using stage-specific antibodies. However, no cell lines are available that show stepwise differentiation from oligodendrocyte progenitors to mature oligodendrocytes. Here we show the
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Hu, Q; Frank, I; Williams, V; Santos, JJ; Watts, P; Griffin, GE; Moore, JP; Pope, M; Shattock, RJ
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Valentina Caldera et al.
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