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MAB316

Sigma-Aldrich

Anti-GABA Antibody, clone 5A9

clone 5A9, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

5A9, monoclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG1

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... GABRA1(2554)

Specificity

Specific for GABA and does not cross-react with other amino acids.

Immunogen

GABA-coupled to BSA with glutaraldehyde.

Application

Anti-GABA Antibody, clone 5A9 detects level of GABA & has been published & validated for use in ELISA, IH & WB.
Immunohistochemistry on frozen sections if perfusion fixed. Glutaraldehyde must be included in fixative with paraffin sections: 1:200-1:400. Typical fixative might be 4% PFA, 1% glutaraldehyde in 0.1M sodium phosphate buffer, pH 7.3 .

Immunoblotting not recommended due to low molecular wgt: 103Da.

Optimal working dilution must be determined by the end user.
Research Category
Neuroscience
Research Sub Category
Neurotransmitters & Receptors

Physical form

Format: Purified
Protein A purified
Purified mouse monoclonal in PBS, pH 7.4, 1% BSA, and 0.1% sodium azide as a preservative.

Storage and Stability

Maintain refrigerated at 2–8°C for up to 6 months.

Analysis Note

Control
Myeloblasts, lymphocytes, erythroblasts

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Alain C Burette et al.
The Journal of comparative neurology, 522(9), 2164-2178 (2014-03-19)
The 53 kDa insulin receptor substrate protein (IRSp53) is highly enriched in the brain. Despite evidence that links mutations of IRSp53 with autism and other neuropsychiatric problems, the functional significance of this protein remains unclear. We used light and electron
GABAergic Interneurons are the Major Postsynaptic Targets of Median Raphe Afferents in the Rat Dentate Gyrus.
Halasy, K., et al.
The European Journal of Neuroscience, 4, 144-153 (1992)
E Szabat et al.
Neuroscience, 47(2), 409-420 (1992-01-01)
A monoclonal antibody, 115AD5, was raised against GABA coupled to bovine serum albumin. The monoclonal antibody 115AD5 also reacted with other GABA-protein conjugates. The specificity of the monoclonal antibody was corroborated by enzyme-linked immunoassay, dot-immunobinding experiments and immunostaining of rat
Identification of major S-nitrosylated proteins in murine experimental autoimmune encephalomyelitis.
Oscar A Bizzozero,Jianzheng Zheng
Journal of Neuroscience Research null
S Westerholz et al.
Neuroscience, 168(2), 573-589 (2010-03-27)
Early in development spontaneous activity modulates survival and connectivity of neurons and thus plays a crucial role in the formation of neural networks. The emergence of synchronous activity in cultured neocortical networks initially is driven by large GABAergic interneurons. Here
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