ABC324

Anti-PDL-1 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): PDL-1 Antibody, PDL-1, Programmed cell death 1 ligand-1, programmed death ligand 1, PD-L1, B7-H1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): immunofluorescence: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• UniProt accession no.: Q9NZQ7
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CD274(29126)

Description

General description

Programmed cell death 1 ligand 1 (PDL-1) is a B7 related protein belonging to the BTN/MOG family. PDL-1 is essential for T-cell proliferation and production of IL10 and IFNG. PDL-1 reduces the secretion of IL-2 and IL-10 from memory T cells by inhibiting cell-mediated immune responses. PDL-1 may be involved in reducing allogenic CD4+ memory T-cell responses to endothelial cells. PDL-1 is highly expressed in heart, skeletal muscle, placenta, and lung and weakly expressed in thymus, spleen, kidney, and liver.

Immunogen

KLH-conjugated linear peptide corresponding to human PDL-1.

Application

Anti-PDL-1 Antibody is a highly specific rabbit polyclonal antibody, that targets Programmed cell death 1 ligand 1 & has been tested in western blotting, IHC (Paraffin) & Immunofluorescence.

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected PDL-1 in human heart tissue.

Immunofluorescence Analysis: 20 μg/mL from a representative lot detected PDL-1 in human heart tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PDL-1 in 15 µg of Raji cell lysate.

Target description

~30 kDa observed. Uniprot describes 2 isoforms at ~20 kDa and 1 at ~33 kDa

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Raji cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2