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Key Documents

AB5380

Sigma-Aldrich

Anti-Nitric Oxide Synthase I Antibody

serum, Chemicon®

Synonym(s):

NOS I, bNOS, nNOS

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

serum

antibody product type

primary antibodies

clone

polyclonal

species reactivity

mouse, sheep, rat, human, monkey

manufacturer/tradename

Chemicon®

technique(s)

immunohistochemistry: suitable
western blot: suitable

NCBI accession no.

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

Gene Information

human ... NOS1(4842)

Specificity

Recognizes Neuronal nitric oxide synthase (nNOS, NOS-I, bNOS). No cross reaction with iNOS and eNOS by immunohistochemistry.

Immunogen

Recombinant human neuronal nitric oxide synthase.

Application

Research Category
Neuroscience
Research Sub Category
Oxidative Stress
This Anti-Nitric Oxide Synthase I Antibody is validated for use in IH, WB for the detection of Nitric Oxide Synthase I.
Western blot: 1:1,000-1:3,000 (ECL).

Immunohistochemistry: 1:1,000-1:2,000 (ABC).

Optimal working dilutions must be determined by end user.

Target description

161 kDa

Linkage

Replaces: AB1552

Physical form

Rabbit Serum. Liquid containing 0.05% sodium azide.
Unpurified

Storage and Stability

Maintain for 6 months at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Analysis Note

Control
Brain tissue

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 1


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Estrogen induces nitric oxide production via activation of constitutive nitric oxide synthases in human neuroblastoma cells.
Xia, Y; Krukoff, TL
Endocrinology null
An association of multiple endocrine neoplasia 2B, a RET mutation; constipation; and low substance P-nerve fiber density in colonic circular muscle.
Sebastian K King, Bridget R Southwell, John M Hutson
Journal of Pediatric Surgery null
In vivo excitation of GABA interneurons in the medial prefrontal cortex through 5-HT3 receptors.
M Victoria Puig,Noemi Santana,Pau Celada,Guadalupe Mengod,Francesc Artigas
Cerebral Cortex (1991)
Distribution of NADPH-diaphorase and nitric oxide synthase reactivity in the central nervous system of the goldfish (Carassius auratus).
Rosa M Giraldez-Perez, Susana P Gaytan, Diego Ruano, Blas Torres, Rosario Pasaro
Journal of Chemical Neuroanatomy null
Rosuvastatin preconditioning provides neuroprotection against spinal cord ischemia in rats through modulating nitric oxide synthase expressions.
Die J, Wang K, Fan L, Jiang Y, Shi Z
Brain Research null

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