612080

TIMP-1, Human Neutrophil Granulocyte

Native TIMP-1 from human granulocyte.

• Synonym(s): Tissue Inhibitor of Metalloproteinase-1
• UNSPSC Code: 12352202

Properties

• Quality Level: 100
• Assay: ≥90% (SDS-PAGE)
• form: liquid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: wet ice
• storage temp.: −70°C

Description

General description

Native TIMP-1 from human granulocyte. Member of a family of inhibitors that participate in the activation and regulation of MMP activity. Forms a non-covalent stochiometric complex with latent and active MMPs. Binds to pro-MMP-9 and MMP-9 via their C-terminal domains.

Native TIMP-1 from human granulocyte. Member of a family of inhibitors that participate in the activation and regulation of MMP activity. Forms a non-covalent stoichiometric complex with latent and active MMPs. Binds to pro-MMP-9 and MMP-9 via their C-terminal domains.

Biochem/physiol Actions

Cell permeable: no

Primary Target
MMP activity

Product does not compete with ATP.

Reversible: no

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In 200 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ^® 35 Detergent, 0.05% NaN₃, pH 7.0.

Preparation Note

Prepared from stimulated neutrophils that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.

Reconstitution

Following initial thaw, aliquot and freeze (-70°C).

Other Notes

Kolkenbrock, H., et al. 1996. Biol. Chem. 377, 529.
Kolkenbrock, H., et al. 1995. Biol. Chem. Hoppe Seyler 376, 495.

Legal Information

Brij is a registered trademark of Croda International PLC

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable