368057

Granzyme B Substrate I, Colorimetric

Colorimetric substrate for the detection of granzyme B and caspase-8 activity.

• Synonym(s): Granzyme B Substrate I, Colorimetric, Ac-IETD- pNA, Caspase-8 Substrate I, Ac-IETD-pNA, Caspase-8 Substrate I
• Empirical Formula (Hill Notation): C₂₇H₃₈N₆O₁₂
• Molecular Weight: 638.62
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• Assay: ≥95% (HPLC)
• form: lyophilized solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: white
• solubility: DMSO: 5 mg/mL
• shipped in: ambient
• storage temp.: −20°C

Description

General description

Colorimetric substrate for detection of granzyme B. Cleavage is monitored colorimetrically at ~405 nm.

Colorimetric substrate for the detection of granzyme B and caspase-8 activity. Cleavage of pNA is monitored colorimetrically at ~405 nm.

Biochem/physiol Actions

Cell permeable: no

Primary Target
Colorimetric substrate for the detection of granzyme B and caspase-8 activity

Product does not compete with ATP.

Reversible: no

Warning

Toxicity: Standard Handling (A)

Sequence

Ac-Ile-Glu-Thr-Asp-pNA

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.

Other Notes

Koeplinger, K.A., et al. 2000. Protein Exp. Purif. 18, 378.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable