343209

Fluorescein Diacetate

Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.

• Synonym(s): Fluorescein Diacetate
• Empirical Formula (Hill Notation): C₂₄H₁₆O₇
• CAS Number: 596-09-8
• Molecular Weight: 416.38
• MDL number: MFCD00005062
• UNSPSC Code: 12352200

Properties

• Quality Level: 100
• form: solid
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; protect from light
• color: colorless to yellow
• solubility: ethanol: soluble
• shipped in: ambient
• storage temp.: 2-8°C
• InChI: 1S/C24H16O7/c1-13(25)28-15-7-9-19-21(11-15)30-22-12-16(29-14(2)26)8-10-20(22)24(19)18-6-4-3-5-17(18)23(27)31-24/h3-12H,1-2H3
• InChI key: CHADEQDQBURGHL-UHFFFAOYSA-N

Description

General description

Stains living cells green. Substrate for esterases. Suitable for use in cell viability assays. Removal of the acetate groups by cellular esterases results in fluorescence in cells with intact membranes.

Substrate for lipases and esterases. Abs. Max: 498 nm.

Warning

Toxicity: Standard Handling (A)

Reconstitution

Unstable in solution. Reconstitute just prior to use.

Other Notes

Clements, K.M., et al. 2001. Osteoarthritis Cartilage9, 499.
Bedner, E., et al. 1998. Cytometry33, 1.
Guilbault, G.G., and Kramer, D.N. 1964. Anal. Chem. 36, 409.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable