1.25251

Chromolith^® Si

L × I.D. 100 mm x 25 mm Prep column

• Synonym(s): Chromolith^® Prep Si 100-25 column
• UNSPSC Code: 41115709

Properties

• material: PEEK column
• Quality Level: 100
• Agency: suitable for USP L3
• product line: Chromolith^®
• feature: endcapped: no
• packaging: 1 ea of
• parameter: 100 bar pressure; 45 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable (Prep LC); UHPLC-MS: suitable; UHPLC: suitable
• column L × I.D.: 10 cm × 2 mm
• matrix: monolithic silica
• matrix active group: Silica bonding phase
• pore size: 12 nm mesoporosity; 3 μm macroporosity
• operating pH range: 2-7.5
• separation technique: hydrophilic interaction (HILIC); normal phase
• storage temp.: no temp limit

Description

General description

Preparative HPLC involves much higher sample loading volumes than analytical chromatography. Consequently, greater sample throughput and separation speed are essential for optimal productivity. These criteria are best fulfilled by Chromolith^® Prep Si columns. The combination of macro and mesopores maximizes separation efficiency and flow rate, while minimizing resistance.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

Analysis Note

Pressure: ≤ 15 bar
Theoretical Plates (N/m) (2-nitroanisol): ≥ 50000
Capacity factor (2-nitroanisol): 1.0 - 2.0
Tailing (USP): 0.90 - 1.45 %
separation factor (2-nitroanisol/nitrobenzene): 3.2 - 4.0

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany