114666
Actinomycin D, Streptomyces sp.
Anti-neoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues.
Synonym(s):
Actinomycin D, Streptomyces sp., Dactinomycin, RNA Polymerase I Inhibitor I, Pol I Inhibitor I
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About This Item
Empirical Formula (Hill Notation):
C62H86N12O16
CAS Number:
Molecular Weight:
1255.42
MDL number:
UNSPSC Code:
12352200
Recommended Products
Quality Level
Assay
≥98% (HPLC)
form
crystalline solid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
protect from light
color
red
solubility
DMSO: 1 mg/mL
chloroform: soluble
methanol: soluble
shipped in
ambient
storage temp.
2-8°C
InChI
1S/C62H86N12O16/c1-27(2)42-59(84)73-23-17-19-36(73)57(82)69(13)25-38(75)71(15)48(29(5)6)61(86)88-33(11)44(55(80)65-42)67-53(78)35-22-21-31(9)51-46(35)64-47-40(41(63)50(77)32(10)52(47)90-51)54(79)68-45-34(12)89-62(87)49(30(7)8)72(16)39(76)26-70(14)58(83)37-20-18-24-74(37)60(85)43(28(3)4)66-56(45)81/h21-22,27-30,33-34,36-37,42-45,48-49H,17-20,23-26,63H2,1-16H3,(H,65,80)(H,66,81)(H,67,78)(H,68,79)
InChI key
RJURFGZVJUQBHK-UHFFFAOYSA-N
General description
Anti-neoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. At higher concentrations, DNA polymerase is inhibited. Also acts as a competitive inhibitor of serine proteases. Inhibits cell growth and colony formation in synchronized HeLa cells. Induces apoptosis in many cell lines. However, actinomycin D has also been shown to suppress etoposide-induced apoptosis in PC12 cells. Note: 1 set = 20 x 200 µg.
Antineoplastic antibiotic. Inhibits DNA-primed RNA polymerase by complexing with DNA via deoxyguanosine residues. A DNA-intercalator with growth inhibitory activity against certain leukemias and sarcomas. At higher concentrations, DNA polymerase is inhibited. Inhibits cell growth and colony formation in synchronized HeLa cells. A potent inducer of apoptosis in many cell lines; however, actinomycin D has also been shown to suppress programmed cell death of PC12 cells induced by etoposide, an inhibitor of topoisomerase II. Acts as a competitive inhibitor of serine protease from microorganisms. The growth of S. aureus is inhibited by 0.25-0.75 µg/ml, whereas a concentration of 100 µg/ml is required to inhibit E. coli due to permeability differences. Heat resistant and stable between pH 2 and 11; decomposed by strong acids and bases. λmax 441 nm ±2 nm. Note: 1 set = 20 vials x 200 µg.
Stability: heat resistant and stable between pH 2 and 11. Decomposed by strong acids and bases.
Biochem/physiol Actions
Primary Target
serine proteases
serine proteases
Secondary Target
cell growth and colony formation in synchronized HeLa cells
cell growth and colony formation in synchronized HeLa cells
Warning
Toxicity: Highly Toxic & Carcinogenic / Teratogenic (I)
Reconstitution
Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short term storage. Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Nakajima, M., et al. 1994. Neurosci. Lett.176, 161.
Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
Yung, B.Y., et al. 1992. Int. J. Cancer52, 317.
Martin, S.J., et al. 1990. J. Immunol.145, 1859.
Yung, B.Y., et al. 1990. Cancer Res.50, 5987.
White, R.J., and Phillips, D.R. 1985. Biochemistry27, 9122.
Madharavao, M., et al. 1978. J. Med. Chem.21, 958.
Sengupta, S.K., et al. 1975. J. Med. Chem.18, 1175.
Wu, M.H., and Yung, B.Y. 1994. Eur. J. Pharmacol. 270, 203.
Betzel, C., et al. 1993. Biochim. Biophys. Acta 1161, 47.
Yung, B.Y., et al. 1992. Int. J. Cancer52, 317.
Martin, S.J., et al. 1990. J. Immunol.145, 1859.
Yung, B.Y., et al. 1990. Cancer Res.50, 5987.
White, R.J., and Phillips, D.R. 1985. Biochemistry27, 9122.
Madharavao, M., et al. 1978. J. Med. Chem.21, 958.
Sengupta, S.K., et al. 1975. J. Med. Chem.18, 1175.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 2 Oral - Carc. 1B - Repr. 1B
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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