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N21588

Sigma-Aldrich

2-Nitrophenylhydrazine

97%

Synonym(s):

(2-Nitrophenyl)hydrazine, (o-Nitrophenyl)hydrazine, 1-(2-Nitrophenyl)hydrazine, 1-Hydrazino-2-nitrobenzene, 2-Hydrazino-1-nitrobenzene, o-(Hydrazino)nitrobenzene

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About This Item

Linear Formula:
O2NC6H4NHNH2
CAS Number:
3034-19-3
Molecular Weight:
153.14
Beilstein:
512797
EC Number:
221-222-6
MDL number:
MFCD00007577
UNSPSC Code:
12352100
PubChem Substance ID:
24897353
NACRES:
NA.22

Assay

97%

form

powder

contains

30% water as stabilizer (minimum)

mp

91-93 °C (lit.)

storage temp.

2-8°C

SMILES string

NNc1ccccc1[N+]([O-])=O

InChI

1S/C6H7N3O2/c7-8-5-3-1-2-4-6(5)9(10)11/h1-4,8H,7H2

InChI key

FRBUNLLUASHNDJ-UHFFFAOYSA-N

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Pictograms

FlameExclamation mark
GHS02,GHS07

Signal Word

Danger

Hazard Statements

H228,H302,H315,H319,H335

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Sol. 1 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

4.1B - Flammable solid hazardous materials

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
MKCQ5196
MKCM3685
MKCL9736
MKCF1038
MKCD8279

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Sigma-Aldrich

109959

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H Miwa et al.
Journal of AOAC International, 79(2), 493-497 (1996-03-01)
Direct derivatization of saponified fats and oils with 2-nitrophenylhydrazine hydrochloride produced corresponding fatty acid hydrazides without time-consuming sample cleanup and/or extraction steps. Hydrazides were injected directly into a liquid chromatograph. Improved isocratic separation was achieved within only 22 min for
Takashi Arai et al.
Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 125(3), 299-305 (2005-03-02)
In general, spectrophotometric methods (inductively coupled plasma or atomic absorption spectrophotometry) are used for the assay of magnesium stearate (Mg-St). In this study, a new rapid, selective assay method was developed for Mg-St in pharmaceutical formulations. The method was based
Junmei Zhang et al.
Journal of the American Society for Mass Spectrometry, 22(11), 1958-1967 (2011-09-29)
Peptides with two or more basic residues, including those with post-translational modifications (PTMs), such as methylation and phosphorylation, can be highly hydrophilic and, therefore, are often difficult to be retained on a reversed-phase (RP) column. In addition, these highly hydrophilic
R Peters et al.
Journal of chromatography. A, 1031(1-2), 35-50 (2004-04-03)
2-Nitrophenylhydrazine (2-NPH) is widely used for the derivatization of carboxylic acids, aldehydes and ketones, in industrial and biological samples. These compounds react with 2-NPH to form derivatives, which are separated by high-performance liquid chromatography (HPLC) and detected with diode array
Determination of methyl ethyl ketone in human urine after derivatization with o-nitrophenylhydrazine, using solid-phase extraction and reversed-phase high-performance liquid chromatography and ultraviolet detection.
J E Van Doorn et al.
Journal of chromatography, 489(2), 419-424 (1989-04-14)
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