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Key Documents

G005946

HPLC Analysis of Proteins on Antibodix® WCX-NP10, Comparison of WCX Columns

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 μm particles (Z777272)
mobile phase: (A) 10 mM sodium phosphate buffer, pH 6.0; (B) A + 1.0 M NaCl
gradient: 0 to 100% B in 42 min
flow rate: 1.0 mL/min
column temp.: ambient
detector: UV, 214 nm
injection: 5 μL
sample: 1 mg/mL each

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

Legal Information

Antibodix is a registered trademark of Sepax Technologies

Analyte

Description
    Worthington Accessories, cap for 4 L Dewar
    Lysozyme from chicken egg white, powder or granules, ≥90 %, ≥39,000 units/mg protein
    Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized

analytical column

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Description
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