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G005699

HPLC Analysis of Fluoroquinolone Antibiotics on Ascentis® Express C18

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 3.0 mm I.D., 2.7 μm particles (53814-U)
mobile phase: [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile; (85:15, A:B)
flow rate: 1.2 mL/min
column temp.: 35 °C
detector: UV, 254 nm
injection: 4 μL
sample: 200 ng/mL in 25:75, water: acetonitrile

suitability

application for HPLC

application(s)

food and beverages
pharmaceutical (small molecule)

Analysis Note

This application demonstrates the suitability of the Ascentis Express C18 for the analysis of fluoroquinolones.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Ofloxacin
    Ciprofloxacin
    Danofloxacin
    Enrofloxacin

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