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G005652

HPLC Analysis of 16 Triazine and Phenyl Urea Compounds on Ascentis® Express RP-Amide, Modified Gradient

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express RP-Amide, 15 cm x 3 mm I.D., 2.7 μm particles (53919-U)
mobile phase: [A] water; [B] acetonitrile
gradient: 10 to 25% B in 30 min; 25 to 60% B in 15 min; held at 60% B for 5 min
flow rate: 0.4 mL/min
column temp.: 35 °C
detector: UV, 240 nm
injection: 5 μL
sample: 20 mg/L each (except terbuthylazin desethyl and terbuthylazine 60 mg/L) in 60:40, water: acetonitrile

suitability

application for HPLC

application(s)

agriculture
chemicals and industrial polymers

Analysis Note

This application demonstrates the suitability of Ascentis Express RP-Amide for the efficient separation of 16 triazine and phenyl urea compounds. Lower column
temperature can improve resolution of the critical pair.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Atrazine-desisopropyl
    Atrazine-desethyl
    Carbendazim
    Terbumeton-desethyl
    Simazine
    Terbuthylazin-desethyl
    Cyanazine
    Atrazine
    Chlortoluron
    Isoproturon
    Terbumeton
    Diuron
    Propazine
    Terbuthylazine
    Linuron
    Terbutryn
    See All (16)

analytical column

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