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G004682

HPLC Analysis of Sugars and Sugar Enantiomers on Astec® CYCLOBOND I 2000

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)
mobile phase: [A] water; [B] acetonitrile; (15:85, A:B)
flow rate: 1 mL/min
column temp.: 35 °C
detector: ELSD at 35°C and 3.2 bar nitrogen
injection: 10 μL
sample: 0.5 mg/mL each in water:acetonitrile, 25:75

suitability

application for HPLC

application(s)

food and beverages

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    D-(−)-Fructose, ≥99% (HPLC), BioReagent, suitable for cell culture, suitable for insect cell culture
    D-(+)-Glucose monohydrate, meets analytical specification of Ph. Eur., BP, Ph Franç., 97.5-102.0% anhydrous basis(HPLC)
    Sucrose, ≥99.5% (GC)
    D-(+)-Maltose monohydrate, ≥99% (HPLC), BioXtra
    Lactose, tested according to Ph. Eur.

analytical column

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standard

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