845161

TOYOPEARL^® AF-rProtein A HC-650F MiniChrom Column

phase rProtein A, 45 μm particle size, pore size 1000 Å, L × I.D. 10 cm × 8 mm, MiniChrom Column

• Synonym(s): MiniChrom
• UNSPSC Code: 41106500

Properties

• material: polyethylene column; polypropylene and polyethylene frit
• product line: TOYOPEARL^®
• specific analyte(s): IgG
• packaging: pkg of 1 ea
• manufacturer/tradename: Tosoh 45161
• parameter: 3 bar max. pressure (resin); 30 bar max. pressure (column)
• technique(s): HPLC: suitable; LPLC: suitable
• L × I.D.: 10 cm × 8 mm
• bed volume: 5 mL
• matrix: hydroxylated methacrylic polymer
• matrix active group: rProtein A phase
• particle size: 45 μm
• pore size: 1000 Å
• operating pH: 2-13
• capacity: >70 g/L adsorption capacity (IgG)(dynamic at 5 min. residence time); >80 g/L adsorption capacity (IgG)(static)
• separation technique: affinity

Description

General description

MiniChrom columns for TOYOPEARL^® and TSKgel^® are pre-packed with the most popular ion exchange, mixed-mode, hydrophobic interaction or affinity media. These columns are available in 5 mL volume, 8 mm ID x 10 cm L, and can be operated with standard laboratory LC systems. MiniChrom columns for TOYOPEARL and TSKgel provide:

• Direct connection to common laboratory LC Systems
• Easy parameter screening and method optimization
• Fast small scale purification
• Reliable and reproducible results

Compatibility

Columns are tolerant to aqueous buffers and salt solutions, 1 mol/L alkaline solutions, 1 mol/L non-oxidizing mineral acids, 8 mol/L urea, 6 mol/L guanidine hydrochloride, non-halogenated organic solvents and detergents. They are not compatible with strong oxidants and halogenated solvents.

Specifications

Clean in place with 0.1 M NaOH.

Physical form

Shipped in 20% (v/v) ethanol.

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Legal Information

TSKgel is a registered trademark of Tosoh Corporation

Toyopearl is a registered trademark of Tosoh Corporation