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Ascentis® Express HILIC, 2 μm UHPLC Column

2 μm particle size, L × I.D. 5 cm × 2.1 mm

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About This Item

UNSPSC Code:
41115700
NACRES:
SB.52

material

stainless steel column

Quality Level

100

Agency

suitable for USP L3

product line

Ascentis®

feature

endcapped: no

manufacturer/tradename

Ascentis®

packaging

1 ea of

parameter

≤60 °C temp. limit
1000 bar max. pressure (14500 psi)

technique(s)

LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable

L × I.D.

5 cm × 2.1 mm

surface area

120 m2/g

impurities

<5 ppm metals

matrix

particle platform (Fused-Core)
superficially porous particle

matrix active group

silica phase

particle size

2 μm

pore size

90 Å pore size

pH range

1-8

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase

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General description

The Ascentis® Express 2μm HILIC or bare silica stationary phase provides a UHPLC column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical reversed-phase mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, or neutral compounds.

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

Lot/Batch Number
USKOP001376
USKOP001371
USKOP001375
USKOP001341
USKOP001337

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Paweł Kubica et al.
Journal of pharmaceutical and biomedical analysis, 127, 184-192 (2016-01-20)
Hydrophilic interaction liquid chromatography (HILIC) coupled with tandem mass spectrometry (MS/MS) was used to separate artificial and natural sweeteners approved for use in European Union (EU). Among three tested HILIC columns (BlueOrchid PAL-HILIC, Ascentis Express Si and Acclaim™ Trinity™ P2)
Tiziana Bertolini et al.
Journal of chromatography. A, 1365, 131-139 (2014-09-23)
A simple, sensitive and fast hydrophilic interaction liquid chromatography (HILIC) method using ultraviolet diode-array detector (UV-DAD)/electrospray ionization tandem mass spectrometry was developed for the automated high performance liquid chromatography (HPLC) determination of sodium risedronate (SR) and its degradation products in
Imran Ali et al.
Biomedical chromatography : BMC, 26(8), 1001-1008 (2012-01-13)
Superficially porous silica particles columns (SPSPCs) are manufactured by different companies. The most common have the brand names Halo, Ascentis Express and Kinetex. These columns provide super fast, sharp peaks and moderate sample loading and back pressure. These are available
Alexandre Grand-Guillaume Perrenoud et al.
Journal of chromatography. A, 1360, 275-287 (2014-08-19)
Superficially porous particles (SPP), or core shell particles, which consist of a non-porous silica core surrounded by a thin shell of porous silica, have gained popularity as a solid support for chromatography over the last decade. In the present study
Jan Soukup et al.
Journal of chromatography. A, 1374, 102-111 (2014-12-30)
Excess adsorption of water from aqueous acetonitrile mobile phases was investigated on 16 stationary phases using the frontal analysis method and coulometric Karl-Fischer titration. The stationary phases include silica gel and silica-bonded phases with different polarities, octadecyl and cholesterol, phenyl

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