ZRB2377
Anti-p-PKCβ (Ser660) Antibody, clone 2C11 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Anti-Ly-6C1 Antibody, clone 2H5 ZooMAb® Rabbit Monoclonal, EC:2.7.11.13, PKC-B, PKC-beta, Protein kinase C beta type
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.43
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2C11, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 76.87 kDa
observed mol wt ~77 kDa
purified by
using Protein A
species reactivity
mouse, monkey
species reactivity (predicted by homology)
rat, rabbit, bovine, human
packaging
antibody small pack of 25
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
inhibition assay: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... PRKCB(5579)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 2C11 is a ZooMAb® rabbit recombinant monoclonal antibody that specifically detects Protein Kinase C β (PKCβ) phosphorylated on serine 660.
Immunogen
KLH-conjugated linear peptide corresponding to 12 amino acids surrounding phosphoserine 660 from the C-terminal region of human Protein Kinase C β (PKCβ).
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PKC β phosphorylated on serine 660 in lysate from NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA; 400 ng/mL; 20 min).
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound p-PKC β (Ser660) peptide with a KD of 9.8 x 10-7 in an affinity binding assay.
Peptide Inhibition Assay: Target band detection in lysate from NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA; 400 ng/mL; 20 min) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-PKCβ (Ser660) in serum starved NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA; 400 ng/ml) for 20 min.).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in lysate from NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA).
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PKC β phosphorylated on serine 660 in lysate from NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA; 400 ng/mL; 20 min).
Tested Applications
Affinity Binding Assay: A representative lot of this antibody bound p-PKC β (Ser660) peptide with a KD of 9.8 x 10-7 in an affinity binding assay.
Peptide Inhibition Assay: Target band detection in lysate from NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA; 400 ng/mL; 20 min) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-PKCβ (Ser660) in serum starved NIH3T3 cells treated with phorbol 12-myristate 13-acetate (PMA; 400 ng/ml) for 20 min.).
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Protein kinase C beta type (UniProt: P05771; also known as EC:2.7.11.13, PKC-B, PKC-beta) is encoded by the PRKCB (also known as PKCB, PRKCB1) gene (Gene ID: 5579) in human. PKCβ is a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine kinase that is involved in multiple cellular processes, including the regulation of B-cell receptor, oxidative stress-induced apoptosis, insulin signaling, and endothelial cell proliferation. PKCβ is comprised of two splice variants, PKCβI and PKCβII, which are from the same gene and differ only in their last 50 amino acids. It binds three calcium ions per subunit and these ions are bound to the C2 domain (aa 158-275). Three specific sites: threonine 500 in the activation loop of the kinase domain; threonine 642 in the turn motif, and serine 661 in the hydrophobic region need to be phosphorylated for its full activation. Phosphorylation on threonine 500 renders it competent for autophosphorylation. Subsequent phosph
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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