ZRB1557
Anti-phospho-GSK3β Ser9 Antibody, clone 6K18 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Glycogen synthase kinase-3 beta
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About This Item
UNSPSC Code:
12352203
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biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
6K18, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 46.74 kDa
observed mol wt ~45 kDa
purified by
using Protein A
species reactivity
human, mouse
species reactivity (predicted by homology)
rat
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
immunocytochemistry: suitable
peptide synthesis: suitable
western blot: suitable
dilution
(Peptide Inhibition assay)
isotype
IgG
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
phosphorylation (pSer9)
Gene Information
human ... GSK3B(2932)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 6K18 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects GSK-3b phosphorylated on serine 9.
Immunogen
KLH-conjugated linear peptide corresponding to 9 amino acids surrounding phosphoserine 9 in human GSK-3b.
Application
Quality Control Testing
Evaluated by Western Blotting in lysate from HeLa cells treated with phorbol 12-myristate 13-acetate (PMA).
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected GSK-3β phosphorylated on serine 9 in lysate from HeLa cells treated with phorbol 12-myristate 13-acetate (PMA; 500 nM; 30 min).
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected phospho-GSK3β Ser9 in lysate from NIH3T3 cells treated with PDGF.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-GSK3β-Ser9 in HeLa cells treated with phorbol 12-myristate 13-acetate (PMA)..
Peptide Inhibition Assay: Target band detection in lysate from HeLa cell treated with phorbol 12-myristate 13-acetate m(PMA) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Evaluated by Western Blotting in lysate from HeLa cells treated with phorbol 12-myristate 13-acetate (PMA).
Western Blotting Analysis (WB): A 1:10,000 dilution of this antibody detected GSK-3β phosphorylated on serine 9 in lysate from HeLa cells treated with phorbol 12-myristate 13-acetate (PMA; 500 nM; 30 min).
Tested applications
Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected phospho-GSK3β Ser9 in lysate from NIH3T3 cells treated with PDGF.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected phospho-GSK3β-Ser9 in HeLa cells treated with phorbol 12-myristate 13-acetate (PMA)..
Peptide Inhibition Assay: Target band detection in lysate from HeLa cell treated with phorbol 12-myristate 13-acetate m(PMA) was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Glycogen synthase kinase-3 beta (UniProt: P49841; also known as EC:2.7.11.26, GSK-3 beta, Serine/threonine-protein kinase GSK3B) is encoded by the GSK3B gene (Gene ID: 2932) in human. GSK-3 is a multifunctional serine/threonine kinase, is a key regulator of numerous signaling pathways during embryogenesis and in metabolic control. Two isoforms of GSK-3 are reported in mammals: a 51 kDa GSK-3a and a 47 kDa GSK-3b. These two isoforms exhibit about 98% homology in their kinase domains but share only about 36% identity in the last 76 C-terminal amino acid residues. GSK-3a contains a glycine-rich extension at its N-terminal. GSK-3 is constitutively active in cells and is regulated through inhibition of its activity. It shows a preference for target proteins that are pre-phosphorylated at a priming residue located C-terminal to the site of GSK-3 phosphorylation. Priming phosphorylation, although not absolutely required, enhances the efficiency of phosphorylation of most GSK-3 substrates. Phosphorylation of a threonine residue in the activation loop (T-loop) is considered to be essential for its activity. The T-loop of GSK-3a is phosphorylated at Tyr279 and GSK-3b at Tyr216, which play a role in forcing open the substrate-binding site of the enzyme. A primary mechanism of GSK3 activity regulation is phosphorylation of N-terminal serine residues (Serine 21 in GSK-3a and serine 9 in GSK-3b). Phosphorylation of these sites is inhibitory to GSK3 kinase activity because the phosphorylated N-terminus acts as a competitive inhibitor for primed substrates. GSK-3b is constitutively active in resting cells and treatment of cells with an agent, such as insulin, causes GSK-3 inactivation through a PI 3-kinase (PI 3-K)-dependent mechanism. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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