ZMS1075
Anti-SARS-CoV-1/2 NP Antibody, clone 1C7C7 ZooMAb® Mouse Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
NC, Nucleocapsid protein, Protein N.
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About This Item
UNSPSC Code:
12352203
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biological source
mouse (recombinant)
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C7C7, recombinant monoclonal
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 45.63 kDa (SARS-CoV-2)
calculated mol wt 46.03 kDa (SARS-CoV)
observed mol wt ~46 kDa
species reactivity
SARS virus
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
flow cytometry: 1 μg/mL
immunocytochemistry: suitable
western blot: 1 μg/mL
isotype
IgG2aκ
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
SARS coronavirus ... N(43740575)
SARS virus ... N(1489678)
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Specificity
Clone 1C7C7 is a ZooMAb mouse recombinant antibody that specifically detects SARS-CoV-1/2 NP.
Immunogen
His-tagged full-length recombinant Nucleoprotein from SARS-CoV virus.
Application
Anti-SARS-CoV-1/2 NP, clone 1C7C7 ZooMAb, Cat. No. ZMS1075, is a Mouse monoclonal antibody that detects nucleoprotein from SARS-CoV-1/2 and is tested for use in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, and Western Blotting.
Flow Cytometry Analysis: 1 mg /ml from a representative lot detected SARS-CoV-1/2 NP in Transfected 293expi cells (Courtesy of Dr Thomas Moran, Center for Therapeutic Antibody Development).
Immunocytochemistry Analysis: 1 μg/mL from a representative lot detected SARS-CoV-1/2 NP in Vero E6 cells infected with SARs-CoV-2 (Courtesy of Dr Ana Fernandez-Sesma Laboratory, Icahn School of Medicine at Mount Sinai (ISMMS), New York).
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SARS-CoV-1/2 NP in lysate from A549 cells infected with SARS-CoV-2 (Courtesy of Jeff Johnson Laboratory, Icahn School of Medicine at Mount Sinai (ISMMS).
Affinity Binding Assay: This antibody bound recombinant nucleoprotein from SARS-CoV-1/2 with a KD of 4.4 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunocytochemistry Analysis: 1 μg/mL from a representative lot detected SARS-CoV-1/2 NP in Vero E6 cells infected with SARs-CoV-2 (Courtesy of Dr Ana Fernandez-Sesma Laboratory, Icahn School of Medicine at Mount Sinai (ISMMS), New York).
Western Blotting Analysis: A 1:5,000 dilution of this antibody detected SARS-CoV-1/2 NP in lysate from A549 cells infected with SARS-CoV-2 (Courtesy of Jeff Johnson Laboratory, Icahn School of Medicine at Mount Sinai (ISMMS).
Affinity Binding Assay: This antibody bound recombinant nucleoprotein from SARS-CoV-1/2 with a KD of 4.4 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Target description
Nucleoprotein (UniProt: P59595/P0DTC9; also known as Nucleocapsid protein, NC, Protein N) is encoded by the N gene (Gene ID: 1489678) in Human SARS coronavirus. The SARS-CoV-2 (COVID-19 causing virus) is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). Nucleoprotein of SARS-CoV and SARS-CoV-2 is a homooligomeric protein that packages the positive strand viral genome RNA into helical ribonucleocapsid and plays a role in virion assembly through its interactions with the viral genome and membrane protein M. It plays an important role in enhancing the efficiency of subgenomic viral RNA transcription as well as viral replication. The RNA binding region of SARS-CoV and SARS-CoV-2 is localized to amino acids 42-187 and 41-186, respectively. Lack of cysteine is one of the common features in nucleoprotein from different coronaviruses. The complete N protein of SARS-CoV and COV-2 is a highly basic with positive net charges and high pI (10.11). Its N-terminal region is more basic with positive charge, while the C-terminal region is acidic with negative charge. The middle region of nucleoprotein displays relatively higher hydrophobicity and the two termini display greater hydrophilicity. Nucleoprotein can undergo phosphorylation at multiple serines by GSK-3, CKII, Akt, CDKs, and Polo-like kinases. Some of these phosphorylation events are essential for RNA binding, oligomerization and localization to nucleoli. Phosphorylation is also required for recruitment of host RNA helicase DDX1 (DEAD-Box Helicase 1) that facilitates template readthrough and enables longer subgenomic mRNA synthesis. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified mouse monoclonal antibody IgG2a in buffer containing PBS, 5% Trehalose without preservatives
Reconstitution
300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.
Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Caleb D Swaim et al.
iScience, 24(10), 103213-103213 (2021-10-12)
The emergence of SARS-CoV-2 has led to a global health crisis that, in addition to vaccines and immunomodulatory therapies, calls for the identification of antiviral therapeutics. The papain-like protease (PLpro) activity of nsp3 is an attractive drug target as it
A serological assay to detect SARS-CoV-2 seroconversion in humans.
Amanat, F., et al.
Nature Medicine, 26, 1033-1036 (2020)
Milan Surjit et al.
Journal of virology, 79(17), 11476-11486 (2005-08-17)
The severe acute respiratory syndrome coronavirus(SARS-CoV) nucleocapsid (N) protein is one of the four structural proteins of the virus and is predicted to be a 46-kDa phosphoprotein. Our in silico analysis predicted N to be heavily phosphorylated at multiple residues.
Michael S Piepenbrink et al.
Cell reports. Medicine, 2(3), 100218-100218 (2021-03-03)
SARS-CoV-2 infection results in viral burden in the respiratory tract, enabling transmission and leading to substantial lung pathology. The 1212C2 fully human monoclonal antibody was derived from an IgM memory B cell of a COVID-19 patient, has high affinity for
Selection, identification, and characterization of SARS-CoV-2 monoclonal antibody resistant mutants.
Fatai S Oladunni et al.
Journal of virological methods, 290, 114084-114084 (2021-01-30)
The use of monoclonal neutralizing antibodies (mNAbs) is being actively pursued as a viable intervention for the treatment of Severe Acute Respiratory Syndrome CoV-2 (SARS-CoV-2) infection and associated coronavirus disease 2019 (COVID-19). While highly potent mNAbs have great therapeutic potential
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