T1299
Anti-Tyrosine Hydroxylase Antibody
mouse monoclonal, TH-2
Synonym(s):
Anti-Tyrosine Hydroxylase, Mouse Anti-Tyrosine Hydroxylase, Tyrosine Hydroxylase Detection
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About This Item
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Product Name
Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse, clone TH-2, ascites fluid
biological source
mouse
Quality Level
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
TH-2, monoclonal
contains
15 mM sodium azide
species reactivity
human, monkey, guinea pig, rat, sheep, bovine, rabbit
technique(s)
immunohistochemistry: suitable
immunoprecipitation (IP): suitable using native or SDS-denatured TH.
western blot: 1:10,000 using extracts of cultured rat adrenal pheochromocytoma (PC12) cells.
General description
Monoclonal Anti-Tyrosine Hydroxylase (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. Tyrosine hydroxylase (TH) RNA undergoes alternative splicing resulting in four isoforms of TH subunit protein in humans. The four human isoforms are TH-1 (55.6 kDa), TH-2 (56 kDa), TH-3 (58.1 kDa), and TH-4 (58.5 kDa). Only humans express all four isoforms.
Tyrosine hydroxylase (TH) is encoded by the gene mapped to human chromosome 11p15. TH is a tetramer of four identical subunits, which is characterized with a regulatory, catalytic, and tetramerization domains. The enzyme utilizes tyrosine, tetrahydrobiopterin (BH4) and O2 as cosubstrates, and Fe2+ as a cofactor.
Specificity
The antibody recognizes an epitope present in the N-terminal region (approx. aa 9-16) of both rodent and human tyrosine hydroxylase. Clone TH-2 reacts with the intact TH subunits.
Immunogen
rat tyrosine hydroxylase (TH).
Application
Monoclonal Anti-Tyrosine Hydroxylase antibody produced in mouse has also been used in
- western blotting
- indirect immunofluorescence
- immunostaining
- immunohistochemistry
- enzyme linked immunosorbent assay (ELISA)
- dot blot
- immunoprecipitation
Monoclonal Anti-tyrosine hydroxylase antibody produced in mouse has been used in western blotting and immunocytochemistry.
Biochem/physiol Actions
Tyrosine hydroxylase (TH) catalyzes the first rate limiting step in the biosynthesis of catecholamine neurotransmitter i.e. the conversion of L-tyrosine to L-dopa. Inhibition of TH by L-phenylalanine, might play a crucial role in phenylketonuria and block the synthesis of norepinephrine. Activity of TH can be regulated by phosphorylation. Decreased expression of TH is associated with various neuropsychiatric diseases such as schizophrenia and Parkinson′s disease (PD).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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recommended
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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