R8653

Anti-ROCK-2 antibody produced in rabbit

~2 mg/mL, affinity isolated antibody, buffered aqueous solution

• MDL number: MFCD07370479
• UNSPSC Code: 12352203
• NACRES: NA.44

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 160 kDa
• species reactivity: human, mouse
• concentration: ~2 mg/mL
• technique(s): immunoprecipitation (IP): 25-50 μg using Jurkat cell lysate; microarray: suitable; western blot: 2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line.
• UniProt accession no.: O75116
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... ROCK2(9475) ; mouse ... Rock2(19878) ; rat ... Rock2(25537)

Description

General description

ROCK2 is a serine/threonine kinase that regulates actin cytoskeletal framework. Absence of ROCK2 alleviates atherosclerosis, whereas its overexpression has been associated with hepatocellular carcinoma. Genetic mutations in ROCK2 has been linked to the risk of Behcet′s disease . Anti-ROCK-2 recognizes human and mouse ROCK-2 (160 kDa).

Immunogen

The sequence is highly conserved in Xenopus ROCK-2.

synthetic peptide corresponding to amino acids 1371-1388 located at the C-terminus of human ROCK-2, conjugated to KLH. This sequence is identical in rat, mouse and bovine ROCK-2.

Application

The antibody is suitable for use in immunoprecipitation (25-50 μg using Jurkat cell lysate), microarray and western blot (2-4 μg/mL using whole cell extract of human acute T cell leukemia Jurkat cell line and of mouse fibroblasts NIH3T3 cell line). The antibody may also be used for immunofluorescence (3-6 μg/ml using NIH3T3 cell line).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable