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MBD0016

Sigma-Aldrich

Inactivated Pseudomonas aeruginosa

Suitable for DNA extraction, PCR, sequencing, next generation sequencing, >10^8 bacteria/ml

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About This Item

UNSPSC Code:
41105500
NACRES:
NA.51

Quality Level

200

form

liquid

concentration

>10^8 bacteria/ml

technique(s)

DNA extraction: suitable
DNA sequencing: suitable
PCR: suitable

shipped in

dry ice

storage temp.

−20°C

General description

Standardization of sample analysis is currently needed in microbiome genomics research workflow. Lack of standardization can lead to biases and errors in common processes during sample preparation and analysis such as sample amplification, sequencing and bioinformatics analyses. Inactivated Pseudomonas Aeruginosa can serve as standard for benchmarking the performance along the workflow of microbiomics or meta-genomics analyses and as a tool to increase reproducibility and allow comparison of results obtained by different labs.

Pseudomonas aeruginosa is a gram negative aerobic to facultative anaerobe rod shaped bacteria which commonly inhabits soil and aqueous environments. In cases of a compromised immune system Pseudomonas aeruginosa can be an opportunistic pathogen. Its devastating effect is mostly known in Cystic Fibrosis (CF) patients. In addition, it often poses a major problem in burn wounds, chronic wounds, and in chronic obstructive pulmonary Disorder (COPD). P.aeruginosa forms biofilms on implanted biomaterials within hospital surface and water supplies. The international nosocomial infection control consortium reported that P. aeruginosa nosocomial infections have become a worldwide healthcare issue.


Read here how to use our standards to ensure data integrity for your microbiome research.

Application

Inactivated bacteria are provided at >10^8 bacteria/mL concentration in DPBS buffer pH 7.2. It is recommended to avoid freeze thaw cycles of this product.
Suitable for Quantitative standard for PCR, Sequencing and NGS

Features and Benefits

  • Individual microbial standard for microbiomics and meta-genomics workflow
  • Suitable standard for PCR, sequencing and NGS
  • Improve Bioinformatics analyses
  • Increases reproducibility
  • Compare results lab to lab

Physical form

Liquid -The inactivated bacteria is provided at >10^8 bacteria/mL in DPBS buffer pH 7.2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Human Microbiome Research Workflow: An Overview of the Role in Human Health and Disease

An overview of human microbiome research, workflow challenges, sequencing, library production, data analysis, and available microbiome reagents to support your research.

DNA Standards for Metagenomic Research into Microbiome Communities

The use of standards is critical to the integrity of metagenomics research. Learn how DNA standards for bacteria, fungi, and viruses are applied to studying the microbiome. Choose standards for E. coli and other key species, as well as mixed community standards.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service