MAK353
Methanol Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
Methyl Alcohol Detection Kit
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About This Item
UNSPSC Code:
12161503
Recommended Products
usage
sufficient for 100 colorimetric tests
detection method
colorimetric
storage temp.
−20°C
General description
Methanol is the simplest alcohol, consisting exclusively of a methyl group and a hydroxyl moiety. It is both an important industrial molecule (solvent, fuel, building block for chemical synthesis) and a biological metabolite; many bacteria generate methanol as a result of anaerobic metabolism. In humans, ingestion of large quantities of methanol is toxic and suppresses the nervous system; this is termed ‘methanol poisoning′. As such, methanol is frequently used as an additive in industrial alcohols to prevent human consumption. The toxicity of methanol is primarily due to the biological product of its metabolism, formaldehyde, which is further metabolized into formic acid. At lower concentrations, methanol poisoning can cause loss of coordination and discomfort, and at higher concentrations will lead to kidney failure, blindness, and even death. In the gut, microbial breakdown of pectin-rich foods produces small amounts of methanol. In addition, some bacteria are capable of metabolizing methane gas, generating methanol, and so monitoring methanol concentration is also relevant for industrial purposes and renewable energy research.
Suitability
Suitable for the determination of methanol concentration in biological samples (serum, plasma and urine), cell and tissue culture supernatants, fruit juice and other non-alcoholic beverages and anaerobic microbial cultures.
Principle
The Methanol Assay Kit utilizes an enzymatic mechanism by which conversion of methanol is correlated stoichiometrically with generation of a colorimetric signal that can be quantified at 450 nm. The assay shows greater than 100-fold specificity for methanol over ethanol and can detect as little as 500 pmol methanol.
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Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Skin Irrit. 2 - STOT SE 1
Target Organs
Eyes
Storage Class Code
3 - Flammable liquids
Flash Point(F)
49.5 °F
Flash Point(C)
9.7 °C
Certificates of Analysis (COA)
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