M041130

6-Fluorescein Phosphoramidite

configured for ABI

• Synonym(s): 6-FAM-phosphoramidite
• Empirical Formula (Hill Notation): C₄₆H₅₈N₃O₁₀P
• CAS Number: 204697-37-0
• Molecular Weight: 843.94
• MDL number: MFCD01940925
• UNSPSC Code: 41116105
• PubChem Substance ID: 329817777
• NACRES: NA.21

Properties

• type: for DNA synthesis
• product line: Proligo Reagents
• Assay: ≥97% (³¹P-NMR); ≥97.0% (reversed phase HPLC)
• color: solid white to off-white
• solubility: acetonitrile: 0.1 M, clear, colorless to faintly yellow (liquid)
• λ: conforms (UV/VIS Identity)
• suitability: conforms to structure for H-NMR
• compatibility: configured for ABI
• storage temp.: −20°C
• SMILES string: CC(C)N(C(C)C)P(OCCCCCCNC(=O)c1ccc2C(=O)OC3(c4ccc(OC(=O)C(C)(C)C)cc4Oc5cc(OC(=O)C(C)(C)C)ccc35)c2c1)OCCC#N
• InChI: 1S/C46H58N3O10P/c1-29(2)49(30(3)4)60(55-25-15-22-47)54-24-14-12-11-13-23-48-40(50)31-16-19-34-37(26-31)46(59-41(34)51)35-20-17-32(56-42(52)44(5,6)7)27-38(35)58-39-28-33(18-21-36(39)46)57-43(53)45(8,9)10/h16-21,26-30H,11-15,23-25H2,1-10H3,(H,48,50)
• InChI key: MGPYJVWEJNTXLC-UHFFFAOYSA-N

Description

General description

Phosphoramidite technology is ideally suited for the modification ofsynthetic oligonucleotides with covalently attached reporter moieties,linkers, spacers, or haptens. Sigma-Aldrich provides popular reporters andlinkers ready to use for DNA/RNA synthesis machines such as β-cyanoethylphosphoramidites. 6-Fluorescein Phosphoramidite is one such label whose absorbance maxima is 496 nm and emission maxima is 520 nm.Key Features of 6-Fluorescein Phosphoramidite:

• 6-Fluorescein Phosphoramidite consists of one isomer derived from6-carboxy fluorescein
• Couples to the 5′-end of the oligonucleotide using standard synthesisprotocols
• Protecting groups on the fluorescein moiety are removed under standardconditions of cleavage and deprotection with concentrated ammonia
• A detritylation step is not required since the fluorescein phosphoramiditedoes not contain a DMT-group
• The labeled oligonucleotide is ready for use in most applications after theevaporation of ammonia. Standard procedures can be used if additionalpurification is required.
• Lot-to-lot consistent purity and performance
• Manufactured under a certified ISO 9001 quality system6-Fluorescein Phosphoramidite is configured for ABI Synthesizers.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable