L9895
Lectin from Pseudomonas aeruginosa
lyophilized powder
Synonym(s):
PA-I
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About This Item
form
lyophilized powder
Quality Level
potency
<15 μg/mL per mL agglutination activity
storage temp.
−20°C
Biochem/physiol Actions
PA-I has a major affinity for D-galactose and its derivatives. PA-I agglutinates all types of human and animal erythrocytes and interacts also with unicellular protozoa, algae and bacteria. PA-I induces mitogenic stimulation in cultured human lymphocytes pretreated with neuraminidase.
Packaging
Package size based on protein content. Vial content based on protein by biuret.
Quality
Highly purified.
Physical form
Lyophilized powder containing approx. 80% protein (biuret), balance primarily sodium citrate, plus traces of calcium chloride, magnesium chloride and manganese chloride
Preparation Note
Purified by affinity chromatography using a modification of Gilboa-Garber.1
Analysis Note
Agglutination activity is expressed in μg/ml and is determined from serial dilutions of a 1 mg/ml solution using phosphate buffered saline, pH 7.0. This activity is the lowest concentration to agglutinate a 2% suspension of neuraminidase treated human blood group A erythrocytes after one hr incubation at 25 °C.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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