L7025

LB Broth with agar (Lennox)

Tablet microbial growth medium

• Synonym(s): LB Agar, LB agar
• UNSPSC Code: 12352200
• NACRES: NA.85

Properties

• grade: for molecular biology
• Quality Level: 200
• sterility: non-sterile
• form: tablet
• technique(s): microbiological culture: suitable
• application(s): food and beverages; microbiology
• storage temp.: room temp
• suitability: nonselective for Escherichia coli; nonselective for coliforms

Description

General description

Lennox LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates in a low-salt formulation. The addition of agar provides a solid medium for microbial growth.

Application

Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added, including those that require low-salt conditions, such as Zeocin^®.

Features and Benefits

Lennox LB tablet with agar provides:

• A budget-friendly alternative to pre-poured plates
• Convenient small package to eliminate weighing
• Fast preparation – no stirring required

Components

13.72g/L Agar
9.14g/L Tryptone
4.57 g/L Yeast Extract
4.57 g/L NaCl
1.6g/L inert binding agents

Reconstitution

Add each 1.68 g tablet to 48.3 mL water (no stirring required). Autoclave for 20 minutes at 121 °C to sterilize. Allow to cool slightly before making additions, such as antibiotics (if desired). Pour into petri dishes and allow to solidify.

Legal Information

Zeocin is a registered trademark of Cayla Sarl

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable